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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30469


    標題: Reversibly Lipidized Interferon-alpha as an Anti-Hepatitis Drug
    作者: Wei-Chiang SHEN
    Liyun Yuan
    Jeffrey Wang
    貢獻者: University of Southern California School of Pharmacy
    Western University of Health Sciences School of Pharmacy
    關鍵字: reversible lipidization
    interferon
    hepatitis
    日期: 2008-07
    上傳時間: 2017-12-04 15:51:04 (UTC+8)
    摘要: Reversible aqueous lipidization (REAL) technologies involve the use of novel lipidizing reagents to modify peptides and proteins reversibly. Previously, we have demonstrated that REAL can increase plasma half-life, enhance oral absorption, prolong the therapeutic effects, and alter both biodistribution and elimination of peptide drugs. Recently, we have extended our investigation of REAL from peptides to proteins. Human interferon-alpha (INF), a 19.2KD protein containing two disulfide bonds (cys1-cys98;cys29-cys138), was reduced and modified with a reversible lipidization agent. The product of the lipidization, REAL-IFN, was homogenous, with four palmitoyl moieties linked to the four Cys residues in the protein molecule via reversible disulfide linkages. The far-UV circular dichroism (CD) spectrum of REAL-IFN was virtually overlapped with that of IFN, indicating that the IFN structure was not altered by the modification. After iv injection in mice at0.1 mg/kg of REAL-IFN activity was rapidly diminished to an undetectable level at 2 hours post IFN injection at the same dose. Unlike IFN or pegylated IFN, PAL-IFN was predominately localized in the liver of treated animals. Evidence suggested that IFN was slowly relesaed from REAL-IFN into bold circulation upon reduction of the disulfide bonds in vivo, possibly in the liver. Furthermore, the liver-targeting effect of PAL-IFN was demonstrated by the observation that the level 2'-5' oligoadenylate synthetase (OAS)expressed in the liver of mice treated with PAL-IFN was significantly higher than treatment with IFN. Therefore, REAL technology provides an alternative to pegylation for improving the therapeutic efficacy of IFN for the treatment of hepatitis.
    關聯: 第五屆海峽化學、生物及材料研討會,起迄日:2008/07/21-2008/07/22,地點:嘉南藥理科技大學
    Appears in Collections:[藥理學院] 2008第五屆海峽化學、生物及材料研討會

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