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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30297


    標題: 評估藥物於治療粥狀動脈硬化的功效之方法
    Method of Assessing Drug Efficacy In Treating Atherosclerosis
    作者: 郭玫君
    吳明娟
    王芷珊
    貢獻者: 生物科技系
    日期: 2016-08-01
    上傳時間: 2017-04-27 15:34:57 (UTC+8)
    摘要: 本發明係有關於一種評估藥物於治療粥狀動脈硬化的功效之方法,係先準備小鼠血管內皮細胞;加入腫瘤壞死因子於小鼠血管內皮細胞作用3~9個小時;再加入待測藥物於小鼠血管內皮細胞作用一段時間;以及利用複數引子組進行反轉?定?聚合??鎖反應(RT-qPCR)以偵測小鼠血管內皮細胞是否表現粥狀動脈硬化相關基因。
    The invention relates to a method of assessing drug efficacy in treating atherosclerosis. The method comprises steps of preparing a mouse vascular endothelial cell; adding a tumor necrosis factor to the mouse vascular endothelial cell for reaction for 3-9 hours; adding a drug to be tested to the mouse vascular endothelial cell for reaction for a period of time; and detecting whether atherosclerosis related genes are expressed in the mouse vascular endothelial cell by using a plurality of primer sets to conduct RT-qPCR.
    關聯: 公開號:201627668、專利編號:I570407
    申請號:104101691
    國際分類:G01N-033/15(2006.01);C12Q-001/68(2006.01);C40B-030/06(2006.01)
    專利公報:14卷15期、44卷5期
    Appears in Collections:[生物科技系(所)] 專利

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