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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30166


    標題: 利用生物轉換製備6-羥基芹菜素之方法
    Method of Manufacturing 6-Hydroxyapigenin By Biotransformation
    作者: 丁秀玉
    貢獻者: 化粧品應用與管理系
    日期: 2015-06-16
    上傳時間: 2016-12-30 17:04:59 (UTC+8)
    摘要: 本發明係有關於一種利用生物轉換製備6-羥基芹菜素(6-Hydroxyapigenin)之方法,其步驟包括:分別由米麴菌(Aspergillus oryzae)與釀酒酵母(Saccharomyces cerevisiae)中擴增CYP57B3與sCPR基因,並將兩基因形成一融合基因;將融合基因接合至pGAPZA載體之中以形成環狀重組質體;再將環狀重組質體置於微生物表達系統(畢赤酵母,Pichiaa pastoris)中,並在含有芹菜素之培養基內培養此微生物表達系統一作用時間;藉此,可製備出6-羥基芹菜素,尤其係經過72小時培養後,可產生產量高達0.22mg/L之6-羥基芹菜素。
    關聯: 專利編號:I507527
    申請號:102145727
    國際分類:C12P-017/06(2006.01);C12N-015/81(2006.01);C12R-001/84(2006.01)
    專利公報:42卷32期
    Appears in Collections:[化妝品應用與管理系(所)] 專利

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