| 摘要: | 許多研究指出高尿酸血症已經成為腎臟病、高血壓等慢性疾病與發炎反應的潛在危險因子，因此尿酸的控制為預防慢性疾病的重要環節之一。本研究目的：白鳳菜煎煮萃取物對於預防高尿酸小鼠之降尿酸作用。本研究使用35隻ICR雄性小鼠，適應一週後分5組進入如下：C組 (正常控制組)、P組 (高尿酸模式組)、A組 (Allopurinol藥物治療組)、GL與GH兩樣品組各為白鳳菜煎煮萃取物100 mg/kg、500 mg/kg。實驗期總共為7天，除了C組注射0.5 % CMC溶液之外，其他組別腹腔注射即氧?酸 (Potassium oxonate, PO) 250 mg/kg連續7天。每日管餵生理食鹽水 (C、P組) 或Allopurinol (A組) 或白鳳菜樣品 (GL、GH組)。動物犧牲後分析項目如下：血漿尿酸濃度 (Uric acid)、尿素氮 (BUN)、肌酸酐 (Creatinine)，肝臟黃嘌呤氧化? (Xanthine Oxidase, XO) 與黃嘌呤去氫? (Xanthine Dehydrogense, XDH) 之酵素活性測定、脂質過氧化指標含量 (TBARS) 以及抗氧化酵素活性等。動物經PO注射後，血漿尿酸值以P組顯著比其他組別高，給予Allopurinol藥物治療的A組則顯著下降，而給予白鳳菜煎煮萃取物之樣品組，無論是GL或GH組的血漿尿酸值皆顯著低於P組 (p<0.05)。肝臟中XO酵素活性僅A組比C、P組顯著下降，樣品組則沒有差異，但在XDH酵素活性P組顯著比其他組別上升。肝臟中GSH含量濃度在樣品組部分皆顯著低於P組。在肝臟中的體內抗氧化酵素活性測定，樣品組皆顯著低於P組。本實驗結果指出給予白鳳菜煎煮萃取物在體內具有降尿酸作用，其降尿酸機制應與抑制肝臟中XDH酵素活性有關。
Background and Purpose: Hyperuricemia has become a risk foctor of kidney disease, hypertension and other chronic diseases. Therefore the control of plasma uric acid levels is important. The purpose of our study, is to investigate whether the hot water extract of Gynura formosana as shows the hypouricemic effect in mice.Methods: 35 three-week-old ICR male mice, after a one week adaptation, were divided into the following 5 groups: C group (normal control group), P group (hyperuricemic modle), A group (Allopurinol treatment group), GL and GH group was tube fed with Gynura formosana extract 100 mg/kg BW, 500 mg/kg BW respectively, group C was injected with 0.5% CMC solution, the other groups are intraperitoneal injectied with PO 250 mg/kg, daily for continous 7 days.Group C and P were tube fed with saline, group A was tube fed with Allopurinol (10 mg/kg) daily once, group GL and GH were tube fed with the test sample. After animal sacrifice, plasma uric acid concentration, urea nitrogen (BUN), creatinine were assayed, The enzyme activtites of xanthine oxidase (XO) and xanthine dehydrogenase (XDH), catalase, Glutathione Peroxidase, superoxide dismutase in liver were also measered. We also measured the levels of lipid peroxidation index (TBARS) in liver.Results: Plasma uric acid levels in P group were significantly higher than other groups, Allopurinol medication and GF exteact feeding were significantly decreased the plasma uric acid levels. Gynura formosana water extact also significantly decreased, the XDH enzyme activity. Liver GSH contents in GL, GH group were significantly lower than that in P group. The activities of CAT, SOD, GPx in GH group were significantly lower than that in P group.Conclusion: The results indicate that Gynura formosana extract shows the hypouricemic effect which could be due to th inhibition of liver XDH activity. |
