舞菇（Grifola frondosa, GF）在中國和日本是一種珍貴藥用菇，被普遍使用在傳統生藥和保健食品上，具有增強免疫力和抗癌的作用。本研究主要目的為 :（i）檢測舞菇中所含的生物活性化合物（EPO）在棕櫚酸（PA）-誘導小鼠C2C12骨骼肌細胞之抗糖尿病和抗發炎功效（ii）分析EPO在PA誘導細胞之胰島素訊息、Akt、PI3K、MAPKs及GLUT-4路徑的影響。結果顯示，EPO從1至5 μM有顯著增加萄糖吸收及增加細胞的生長。5 μM EPO會顯著增加葡萄糖吸收和調降PA誘導細胞ROS形成作用。PA誘導結果顯示其會降低細胞存活率至80％，而EPO具有保護細胞活性的作用，使得細胞存活率達100%。細胞加入PA再以5 μM的EPO處理，會顯著增加PI3K的表現，磷酸化Akt，和GLUT-4蛋白質表現和降低磷酸化的JNK和p38蛋白質表現。由上述結果證實，EPO可顯著提高葡萄糖吸收效率及增強胰島素訊息，Akt/PI3K、MAPKs及GLUT-4訊息傳導路徑以及抑制ROS的產生。因此，EPO具有抗糖尿病和抗發炎作用。 Grifola frondosa (GF), a high value medicinal mushroom in China and Japan, is popularly consumed as traditional medicines and health foods for anticancer and enhancing immune functions. In this study, our aims were (i) to examine the anti-diabetic and anti-inflammatory effects of a GF bioactive compound, ergosterol peroxide (EPO) in palmitic acid (PA)-induced murine C2C12 skeletal muscle cells, and (ii) examine the effects of EPO on insulin, Akt/PI3K, MAPKs and GLUT-4 signaling pathways in PA-induced cells. At 1 to 5 ?M significantly up-regulated the glucose absorption and increased the cell growth. 5 ?M EPO significantly up-regulated the glucose absorption and down-regulated ROS formation in PA-induced cells. Results on EPO is effect on PA-stimulated cell function revealed that cell viability after PA-stimulated reduced to 80% while EPO protected the cells to 100%. Co-treatment of cells with PA plus 5 ?M EPO significantly up-regulated the expression of p-IRS-1, PI3K, p-Akt, and GLUT-4 proteins and down-regulated the expression the activation and phosphorylation of JNK and p38 proteins. These results suggest that EPO may play a potential role in the anti-diabetic and anti-inflammatory effects through up-regulation of glucose up-take, insulin, Akt/PI3K, MAPKs and GLUT-4 signaling pathways and inhibiting the production of ROS.
