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|標題: ||6-Shogaol Induces cell cycle arrest and apoptosis in human hepatoma cells through pleiotropic mechanisms|
|作者: ||Wu, Jung-Ju|
Omar, Hany A.
Cell cycle arrest
Reactive oxygen species
Endoplasmic reticulum stress
|上傳時間: ||2016-04-19 19:07:04 (UTC+8)|
|出版者: ||Elsevier Science Bv|
|摘要: ||Shogaols are a group of the active constituents of ginger that have been identified to have various biological activities. The aim of the current study was to investigate the antitumor activity of 6-shogaol in hepatocellular carcinoma (HCC) and the possible involvement of reactive oxygen species as a putative mechanism of action. HCC cell lines, HepG2 and Huh-7, were used to study the in vitro anti-cancer activity of 6-shogaol via the application of various molecular biology techniques. Results showed that 6-shogaol effectively inhibited the cell viability, caused cell cycle arrest at G2/M phase and induced apoptosis in HCC cells as indicated by MIT assay, DAPI nuclear staining, annexin V assay, cell cycle analysis, and activation of caspase-3. Western blot analysis revealed the ability of 6-shogaol to target cancer survival signaling pathways mediated by mitogen-activated protein kinase (MAPK), 5 AMP-activated protein kinase (AMPK) and Akt. In addition, 6-Shogaol induced alteration of cyclin proteins expression and caused cleavage of protein kinase C delta. Furthermore, 6-Shogaol was able to induce the production of reactive oxygen species and encloplasmic reticulum (ER) stress-associated proteins and the consequent activation of autophagy in HepG2 cells. Taken together, the current study highlights evidences that 6-shogaol induces apoptosis, modulates cyclins expression and targets cancer survival signaling pathways in HCC cell lines, at least in part, via the production of reactive oxygen species. These findings support 6-shogaol's clinical promise as a potential candidate for HCC therapy. (C)) 2015 Elsevier B.V. All rights reserved|
|關聯: ||European Journal of Pharmacology, v.762, pp.449-458|
|Appears in Collections:||[藥學系(所)] 期刊論文|
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