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    標題: 6-Shogaol Induces cell cycle arrest and apoptosis in human hepatoma cells through pleiotropic mechanisms
    作者: Wu, Jung-Ju
    Omar, Hany A.
    Lee, Ying-Ray
    Teng, Yen-Ni
    Chen, Pin-Shern
    Chen, Yu-Chung
    Huang, Hsiao-Shan
    Lee, Kuan-Han
    Hung, Jui-Hsiang
    貢獻者: 生物科技系
    藥學系暨藥物科技研究所
    關鍵字: 6-Shogaol
    Apoptosis
    Cell cycle arrest
    Reactive oxygen species
    Autophagy
    Endoplasmic reticulum stress
    Mitogen-actwated protein
    日期: 2015-09
    上傳時間: 2016-04-19 19:07:04 (UTC+8)
    出版者: Elsevier Science Bv
    摘要: Shogaols are a group of the active constituents of ginger that have been identified to have various biological activities. The aim of the current study was to investigate the antitumor activity of 6-shogaol in hepatocellular carcinoma (HCC) and the possible involvement of reactive oxygen species as a putative mechanism of action. HCC cell lines, HepG2 and Huh-7, were used to study the in vitro anti-cancer activity of 6-shogaol via the application of various molecular biology techniques. Results showed that 6-shogaol effectively inhibited the cell viability, caused cell cycle arrest at G2/M phase and induced apoptosis in HCC cells as indicated by MIT assay, DAPI nuclear staining, annexin V assay, cell cycle analysis, and activation of caspase-3. Western blot analysis revealed the ability of 6-shogaol to target cancer survival signaling pathways mediated by mitogen-activated protein kinase (MAPK), 5 AMP-activated protein kinase (AMPK) and Akt. In addition, 6-Shogaol induced alteration of cyclin proteins expression and caused cleavage of protein kinase C delta. Furthermore, 6-Shogaol was able to induce the production of reactive oxygen species and encloplasmic reticulum (ER) stress-associated proteins and the consequent activation of autophagy in HepG2 cells. Taken together, the current study highlights evidences that 6-shogaol induces apoptosis, modulates cyclins expression and targets cancer survival signaling pathways in HCC cell lines, at least in part, via the production of reactive oxygen species. These findings support 6-shogaol's clinical promise as a potential candidate for HCC therapy. (C)) 2015 Elsevier B.V. All rights reserved
    關聯: European Journal of Pharmacology, v.762, pp.449-458
    顯示於類別:[藥學系(所)] 期刊論文
    [生物科技系(所)] 期刊論文

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