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    標題: Argininosuccinate synthetase 1 suppression and arginine restriction inhibit cell migration in gastric cancer cell lines
    作者: Shan, Yan-Shen
    Hsu, Hui-Ping
    Lai, Ming-Derg
    Yen, Meng-Chi
    Chen, Wei-Ching
    Fang, Jung-Hua
    Weng, Tzu-Yang
    Chen, Yi-Ling
    貢獻者: 老人服務事業管理系
    關鍵字: Epithelial Ovarian-cancer
    Hepatocellular-Carcinoma
    Deiminase Treatment
    Signal Transducer
    Nitric-Oxide
    Tumor-Growth
    In-Vitro
    Expression
    Deprivation
    Stat3
    日期: 2015-04
    上傳時間: 2016-04-19 19:05:02 (UTC+8)
    出版者: Nature Publishing Group
    摘要: Gastric cancer metastasis remains a major cause of cancer-related deaths. There is an urgent need to develop new therapeutic approaches targeting metastatic gastric cancer. Argininosuccinate synthetase 1 (ASS1) expression is increased in gastric cancer. We detected the protein expression of ASS1 in human gastric cancer cell lines (AGS, NCI-N87, and MKN45) and in murine gastric cancer cell lines (3I and 3IB2). We used vector-mediated short hairpin RNA(shRNA) expression to silence ASS1 expression in the MKN45 and 3IB2 cell lines, and analyzed the effects of this protein on cell migration and metastasis. We demonstrated that ASS1 silencing suppressed cell migration in the MKN45 and 3IB2 cell lines. ASS1 knockdown significantly reduced liver metastasis in mice after the intrasplenic implantation of 3IB2 cancer cell clones. To determine whether arginine restriction may represent a therapeutic approach to treat gastric cancer, the sensitivity of tumor cells to arginine depletion was determined in gastric cancer cells. Arginine depletion significantly inhibited cell migration in the gastric cancer cell line. The silencing of ASS1 expression in MKN45 and 3IB2 gastric cancer cells markedly decreased STAT3 protein expression. In conclusion, our results indicate that the ASS1 protein is required for cell migration in gastric cancer cell lines.
    關聯: Scientific Reports, v.5 , Article ID 9783
    顯示於類別:[老人服務事業管理系] 期刊論文

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