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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/29685


    標題: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O-Methyltransferase SpOMT2884 from Streptomyces peucetius
    作者: Chiang, Chien-Min
    Ding, Hsiou-Yu
    Tsai, Ya-Ting
    Chang, Te-Sheng
    貢獻者: 生物科技系
    化粧品應用與管理系
    關鍵字: 7,4'-dihydroxy-8-methoxy-isoflavone
    8,4'-dihydroxy-7-methoxy-isoflavone
    8-hydroxydaidzein
    melanogenesis
    inhibition
    o-methyltransferase
    spomt2884
    日期: 2015-11
    上傳時間: 2016-04-19 19:04:33 (UTC+8)
    出版者: Mdpi Ag, Postfach
    摘要: Biotransformation of 8-hydroxydaidzein by recombinant Escherichia coli expressing O-methyltransferase (OMT) SpOMT2884 from Streptomyces peucetius was investigated. Two metabolites were isolated and identified as 7,4-dihydroxy-8-methoxy-isoflavone (1) and 8,4-dihydroxy-7-methoxy-isoflavone (2), based on mass, 1H-nuclear magnetic resonance (NMR) and 13C-NMR spectrophotometric analysis. The maximum production yields of compound (1) and (2) in a 5-L fermenter were 9.3 mg/L and 6.0 mg/L, respectively. The two methoxy-isoflavones showed dose-dependent inhibitory effects on melanogenesis in cultured B16 melanoma cells under non-toxic conditions. Among the effects, compound (1) decreased melanogenesis to 63.5% of the control at 25 M. This is the first report on the 8-O-methylation activity of OMT toward isoflavones. In addition, the present study also first identified compound (1) with potent melanogenesis inhibitory activity.
    關聯: International Journal of Molecular Sciences, v.16 n.11, pp.27816-27823
    Appears in Collections:[化妝品應用與管理系(所)] 期刊論文
    [生物科技系(所)] 期刊論文

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