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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/29681


    標題: Identification of 3 '-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris
    作者: Ding, Hsiou-Yu
    Chiang, Chien-Min
    Tzeng, Wei-Ming
    Chang, Te-Sheng
    貢獻者: 化粧品應用與管理系
    生物科技系
    關鍵字: 3′-Hydroxygenistein
    Genistein
    Melanogenesis
    Tyrosinase
    Inhibition
    Pichia pastoris
    日期: 2015-10
    上傳時間: 2016-04-19 19:04:25 (UTC+8)
    出版者: Elsevier Sci Ltd
    摘要: A product resulting from the biotransformation of genistein by a recombinant Pichia pastoris was isolated and identified as 3'-hydroxygenistein, on the basis of mass, H-1 NMR, and C-13 NMR spectrophotometric analysis. The maximal product concentration and the conversion yield of the biotransformation in a 51 fermenter were 3.5 mg/1 and 14%, respectively. The inhibitory effects of 3'-hydroxygenistein on tyrosinase activity were investigated in vitro using mushroom tyrosinase. The results showed that 3'-hydroxygenistein potently inhibited tyrosinase activity with an IC50 value of 15.9 mu M. Furthermore, the inhibitory effects of 3'-hydroxygenistein on melanogenesis were also investigated in vitro in cultured B16 melanoma cells, and it was shown that 3'-hydroxygenistein dose-dependently inhibited melanogenesis in non-toxic concentrations. In summary, the 3'-hydroxygenistein that was produced from genistein by the recombinant yeast was confirmed as a potent tyrosinase inhibitor and inhibited melanogenesis in B16 cells. (C) 2015 Elsevier Ltd. All rights reserved.
    關聯: Process Biochemistry, v.50 n.10,pp.1614-1617
    Appears in Collections:[生物科技系(所)] 期刊論文
    [化妝品應用與管理系(所)] 期刊論文

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