Djulis (Chenopodiun formosaneum) and its bioactive compounds protect against oxidative stress in human HepG2 cells

doi:10.1016/j.jff.2015.06.025

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標題:	Djulis (Chenopodiun formosaneum) and its bioactive compounds protect against oxidative stress in human HepG2 cells
作者:	Chyau, Charng-Cherng Chu, Chin-Chen Chen, Shih-Ying Duh, Pin-Der
貢獻者:	休閒保健管理系保健營養系食品科技系
關鍵字:	Apoptosis Betanin Cytoprotection Djulis(Chenopodiun formosaneum) Oxidativ estress Rutin
日期:	2015-10
上傳時間:	2016-04-19 19:04:11 (UTC+8)
出版者:	Elsevier Science Bv
摘要:	The effect of water extracts of Djulis (Chenopodiun formosaneum) (WECF) and its bioactive compounds on the oxidative stress of tert-butyl hydroperoxide (t-BHP)-induced HepG2 cells is investigated. WECF (at 10-250 mu g/ml) demonstrates a cytoprotection in 0.2 mM t-BHP-induced HepG2 cells. The oxidative damage was accompanied by depletion of glutathione (GSH), thiobarbituric acid reactive substances (TBARS) formation and reactive oxygen species (ROS) generation which was significantly reduced by WECF. There was a down-regulated degradation of I kappa B-alpha and anti-apoptosis in t-BHP-induced cells by WECF. The increase in the Bcl-2/Bax ratio and the mitochondrial membrane potential and a decrease in caspase-3 activity and the down-modulated degradation of poly ADP-ribose polymerase (PARP) by WECF in t-BHP-induced cells parallels the effect on cell viability. The anti-apoptosis in t-BHP-induced cells by WECF was verified by observation using DAPI staining assay. According to HPLC-DAD and HPLC-MS/MS analysis, betanin, rutin, kaempferol and another twenty compounds were present in WECF, which may partially be responsible for its protective effect against oxidative damage. Collectively, WECF consisting of bioactive compounds can prevent oxidative stress by enhancing the antioxidant indices and reducing apoptosis of cells.
關聯:	Journal of Functional Foods, v.18 Part A, pp.159-170
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