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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/29652


    標題: Early identification of microorganisms in blood culture prior to the detection of a positive signal in the BACTEC FX system using matrix-assisted laser desorption/ionization-time of flight mass spectrometry
    作者: Wang, Ming-Cheng
    Lin, Wei-Hung
    Yan, Jing-Jou
    Fang, Hsin-Yi
    Kuo, Te-Hui
    Tseng, Chin-Chung
    Wu, Jiunn-Jong
    貢獻者: 生物科技系
    關鍵字: Blood culture system
    Blood stream infection
    Mass spectrometry
    Matrix-assisted laserdesorpt ionionization-time of flight mass spectrometry
    Time to detection
    日期: 2015-08
    上傳時間: 2016-04-19 19:03:23 (UTC+8)
    出版者: Elsevier Taiwan
    摘要: Background: Matrix-assisted laser desorption/ionizationetime of flight mass spectrometry (MALDI-TOF MS) is a valuable method for rapid identification of blood stream infection (BSI) pathogens. Integration of MALDI-TOF MS and blood culture system can speed the identification of causative BSI microorganisms.Materials and methods: We investigated the minimal microorganism concentrations of common BSI pathogens required for positive blood culture using BACTEC FX and for positive identification using MALDI-TOF MS. The time to detection with positive BACTEC FX and minimal incubation time with positive MALDI-TOF MS identification were determined for earlier identification of common BSI pathogens.Results: The minimal microorganism concentrations required for positive blood culture using BACTEC FX were > 10(7)-10(8) colony forming units/mL for most of the BSI pathogens. The minimal microorganism concentrations required for identification using MALDI-TOF MS were > 107 colony forming units/mL. Using simulated BSI models, one can obtain enough bacterial concentration from blood culture bottles for successful identification of five common Gram-positive and Gram-negative bacteria using MALDI-TOF MS 1.7-2.3 hours earlier than the usual time to detection in blood culture systems.Conclusion: This study provides an approach to earlier identification of BSI pathogens prior to the detection of a positive signal in the blood culture system using MALDI-TOF MS, compared to current methods. It can speed the time for identification of BSI pathogens and may have benefits of earlier therapy choice and on patient outcome. Copyright (C) 2013, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.
    Appears in Collections:[生物科技系(所)] 期刊論文

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