在過去與大豆異黃酮相關的文獻中發現，在大鼠模型中大豆異黃酮不僅能預防骨質疏鬆症，還能增加體內的抗氧化能力。而黑豆 (Glycine max (L.) Merrill) 由美梭公司之高效低溫萃取設備 (Room Temature Su Extraction System, RTSES) 於黑豆仁、黑豆皮個別萃取後額外添加與混合而成，並在大鼠飲食中進行探討。
在本研究中，我們評估在去卵巢大鼠(OVX) 身上，黑豆萃取物(BSE) 配方對骨骼以及四氯化碳誘導肝臟損傷的影響。將三個月齡的 Sprague-Dawley 大鼠區分成假手術組與去卵巢組，並持續給予黑豆萃取物配方 100 天。各組每公斤體重 ( kg b.w.) 餵食 BSE 含量如下：假手術組餵食 BSE 0 g (C 組) 以及 5 g (BHS 組) ；去卵巢組餵食 BSE 0 g (N 組)，1.25 g (BL 組)，2.5 g (BM 組)，5 g (BH 組)，以及每週管餵 1.5 mg 雌激素 (E 組)。在第 100 天，去卵巢大鼠於腹腔注射四氯化碳 (0.5 ml/ kg b.w.)。以血清骨鈣素 (OCN) 和鹼性磷酸酶 (ALP) 作為骨骼生化指標，骨骼的組織型態測量，肝臟內抗氧化酵素，以及對大鼠淋巴細胞的抗氧化能力與肝臟組織病理切片做測定。其結果顯示，骨骼檢測數據、血清 OCN 和 ALP 濃度，BH 組皆顯著高於 N 組。此外，BH 組顯著改善肝臟抗氧化酵素、淋巴細胞的氧化以及減少肝臟的損傷。綜合上述結果，餵食 BSE 能保護去卵巢大鼠骨骼流失，並可能有助於減少四氯化碳誘導的肝臟損傷。 Soy isoflavones have been reported to prevent bone loss of osteoporosis and increase antioxidative capacity in rat models. However, the effect of extract from black soybeans (Glycine max (L.) Merrill) by room temature su extraction system (RTSES) technique that people consume in their diet needs to be explored. In the present study, we evaluated the effects of black soybeans extract (BSE) on bone biomarkers and hepatoprotection for CCl4-induced liver injury in ovariectomized (OVX) rats. Three-month-old Sprague-Dawley rats were either sham-oated or ovariectomized and then fed diets containing BSE for 100 days. The amounts of BSE ( kg b.w.) in each group were as follows: ovary intact with feeding 0 g (C) and 5 g black soybeans extract (BHS); ovariectomy with feeding 0 (N), 1.25 (BL), 2.5 (BM), 5 (BH) black soybeans extract; and ovariectomy with orally gavaged 1.5 mg estrogen each week (E). In the 100th day, the rats were intraperitoneally injected with CCl4 (0.5 ml/ kg b.w.). Bone biochemical markers in serum-osteocalcin (OCN) and alkaline phosphatase (ALP), histomorphometry for the femur rigidity, histological architecture and various antioxidant enzymes in liver, and antioxidative capacity on lymphocyte of the rat were measured. The results showed that femur rigidity, serum OCN and ALP concentrations were significantly higher in the BH group than the N group. Moreover, BH group had significantly improved liver antioxidant enzymes, oxidation on lymphocyte and reduce the damage of liver. In conclusion, consumption of BSE had a definite protective effect on bone loss in OVX rats, and may help decrease hepatic damage by CCl4-induced injury.
