中文摘要 本研究主要以氮-異丙基丙烯醯胺單體 (N–isopropyl acrylamide 簡稱NIPAAm) 與交聯劑氮-羥甲基丙烯醯胺 [N-(hydroxymethyl) acrylamide 簡稱 NMA] 利用溶液聚合法合成具有溫度感應型之poly(NIPAAm-NMA) 智慧型高分子材料，反應7.5小時測得最終轉化率為94.7%為合成條件。將高分子材料與幾丁聚醣混合，經由一種特殊紡絲法將高分子混合液製成具有生物相容性的奈米纖維，以硼氫化鈉作為還原劑，還原硝酸銀形成銀粒子，將銀粒子加入高分子混合液中，再以特殊紡絲法製成含有銀粒子的奈米纖維，由於奈米纖維含有幾丁聚醣與銀粒子，因此奈米纖維將具有生物相容性及殺菌性。銀粒子來做抑制菌生長的試驗結果。發現不含銀粒子之CA8對於大腸桿菌無法有效達到抑制效果，相對含銀粒子之AM5、APM55、APC58來說卻有明顯達到抑制效果，皆能有效達到控制菌的滋長，最後利用穿透式電子顯微鏡( TEM )、掃描式電子顯微鏡( SEM )觀察各種實驗變因對於奈米纖維型態結構之影響，將不同條件所製得的奈米纖維進行抗菌實驗，探討不同成分的奈米纖維對抗菌功效的影響。關鍵字: 氮-異丙基丙烯醯胺、氮-羥甲基丙烯醯胺、電紡絲、幾丁聚 AbstractIn this study, the thermo-sensitive poly(N–isopropylacrylamide-co-N-(hydroxymethyl) acrylamide) (poly(NIPAAm-NMA)) copolymer were synthesized by the method of solution polymerization. The poly(NIPAAm-NMA) polymer and chitosan were used as materials to manufacture the nano-fiber with the properties of thermo-sensitive and bactericidal by the method of electrospun. Besides, the Ag nano-particles were mixed with the polymer solution to produce the bactericidal nano-fiber. We found the CA8 can not effectively inhibit but the others are effectively inhibit the growth of bacteria reach the antibacterial effect.The effects of various variables on the morphology of nano-fiber were study by the TEM and SEM. Moreover, the compositions of the nano-fiber influenced on the bactericidal property was also studied. Key words: N–isopropylacrylamide, Electrospun, thermo-sensitive Chitosan, Silver particles
