中草藥對脂多醣刺激RAW 264.7巨噬細胞抗發炎反應之研究

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標題:	中草藥對脂多醣刺激RAW 264.7巨噬細胞抗發炎反應之研究 Anti-Inflammatory Effects of Chinese Herbal Medicines in Lipopolysaccharide-Induced Inflammatory Response in RAW 264.7 Macrophages
作者:	張愫菱
貢獻者:	藥學系黃秀琴
關鍵字:	RAW264.7細胞株抗發炎反應中草藥 Anti-inflammatory Traditional Chinese Medicine RAW 264.7 cell line
日期:	2015
上傳時間:	2015-10-21 17:04:32 (UTC+8)
摘要:	本研究選取七種民間常用之具抗發炎中草藥，包括艾草、咸豐草、夏枯草、穿心蓮、荊芥、蒼朮及白芍等，以甲醇萃取所得甲醇萃取物進行細胞毒性試驗、NO及TNF-α細胞激素生成抑制效果、巨噬細胞吞噬試驗及iNOS 蛋白質表現，了解此七種甲醇萃取物之抗發炎效果。結果顯示，七種中草藥經甲醇萃取，分別以蒼朮 (ALM) 萃取率為最高為 11.4%，以艾草 (AAM) 萃取率為最低為 3.2%；七種甲醇萃取物對RAW264.7細胞毒性試驗，分別以穿心蓮甲醇萃取物 (APM) 在0.5 mg/ml和1 mg/ml的濃度下，對脂多醣誘導發炎反應之RAW264.7細胞株存活率分別為73.8% 和65.2%，其細胞存活率小於80%，具有細胞毒性，其他甲醇萃取物皆不具毒性，七種甲醇萃取物對RAW264.7受脂多醣誘發細胞生成細胞激素NO，以艾草甲醇萃取物 (AAM) 在 1 mg/ml的濃度下具有59.6% 最佳的NO生成抑制率；七種甲醇萃取物對RAW264.7受脂多醣誘發生成細胞激素TNF-α，以艾草甲醇萃取物 (AAM) 及咸豐草甲醇萃取物 (BPM) 在1 mg/ml的濃度下分別有69.7% 及 69.4% 最佳的TNF-α生成抑制率；七種甲醇萃取物對RAW264.7吞噬作用，在1 mg/ml的濃度下，皆具有促進吞噬效果，以蒼朮甲醇萃取物 (ALM) 吞噬效果為最佳42.2%；七種甲醇萃取物對RAW264.7受脂多醣誘發生成iNOS 蛋白質表現，皆具有抑制效果。綜合以上結果顯示，七種甲醇萃取物中，以艾草對RAW264.7細胞不具無毒性，且具有抑制細胞激素生成，其有效成分有待進一步探討。 This study selected seven Traditional Chinese Medicines (TCMs) including Artemisia argyi Levl. et Vant. (AA), Bidens pilosa var. radiate Sherff. (BP), Prunella vulgaris L. (PV), Andrographis paniculata (Burm. f.) Nee (AP), Schizonepeta tenuifolia Briq (ST), Atractylodes lancea (Thunb.) DC. (AL) and Paeonia lactiflora Pall. (PL). Seven TCMs was extracted with methanol and obtained seven TCMs methanol extracts. Every TCMs methanol extract was labeled with M. The cytotoxicity test, NO inhibition, TNF-α generation, phagocytic test and iNOS expression were used to evaluate the anti-inflammatory properties of these seven TCMs methanol extracts. The results showed that the highest yield of methanol extract was ALM (11.4%) and the lowest one was AAM (3.2%). Two concentrations (0.5 mg/mL and 1 mg/mL) of APM added into LPS induced RAW 264.7 showed 73.8% and 65.2% of cell viabilities, respectively, indicating that a minor cell toxicity on APM was observed. 1 mg/mL of APM and AAM added into LPS-induced RAW264.7 cells revealed the 60.3% and 59.6% inhibition on NO generation. In addition, 1 mg/mL of AAM and BPM exhibited 69.7% and 69.4% of TNF-inhibition. Overall, seven selected TCMs methanol extracts can promote the phagocytic effect but also inhibit iNOS expression; in particular, ALM exhibited 42.2% best result on promotion of phagocytosis. Based on above test result, AAM had the best anti-inflammatory effect and no toxicity to cell and active constituents of the AAM will be studied.
關聯:	校外公開：2020-09-01,校內公開：2015-09-10，學年度：103，66頁
顯示於類別:	[藥學系(所)] 博碩士論文

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