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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/28603


    標題: Induction of Apurinic Endonuclease 1 Overexpression by Endoplasmic Reticulum Stress in Hepatoma Cells
    作者: Cheng, Tsung-Lin
    Chen, Pin-Shern
    Li, Ren-Hao
    Yuan, Shyng-Shiou
    Su, Ih-Jen
    Hung, Jui-Hsiang
    貢獻者: 生物科技系
    新藥創建研究中心
    關鍵字: hepatocellular carcinoma
    Huh-7
    HepG2
    NeHepLxHT
    hepatitis B virus
    hepatitis B virus pre-S2 Delta large mutant surface protein
    endoplasmic reticulum stress
    apurinic endonuclease 1
    GRP78
    日期: 2014-07
    上傳時間: 2015-05-06 21:22:11 (UTC+8)
    出版者: Mdpi Ag
    摘要: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with poor prognosis due to resistance to conventional chemotherapy and limited efficacy of radiotherapy. Previous studies have noted the induction of endoplasmic reticulum stress or apurinic endonuclease 1 (APE1) expression in many tumors. Therefore, the aim of this study was to investigate the relationship between endoplasmic reticulum (ER stress) and APE1 in hepatocellular carcinoma. Here we investigate the expression of APE1 during ER stress in HepG2 and Huh-7 cell lines. Tunicamycin or brefeldin A, two ER stress inducers, increased APE1 and GRP78, an ER stress marker, expression in HepG2 and Huh-7 cells. Induction of APE1 expression was observed through transcription level in response to ER stress. APE1 nuclear localization during ER stress was determined using immunofluorescence assays in HepG2 cells. Furthermore, expression of Hepatitis B virus pre-S2 Delta large mutant surface protein (pre-S2 Delta), an ER stress-induced protein, also increased GRP78 and APE1 expression in the normal hepatocyte NeHepLxHT cell line. Similarly, tumor samples showed higher expression of APE1 in ER stress-correlated liver cancer tissue in vivo. Our results demonstrate that ER stress and HBV pre-S2 Delta increased APE1 expression, which may play an important role in resistance to chemotherapeutic agents or tumor development. Therefore, these data provide an important chemotherapeutic strategy in ER stress and HBV pre-S2 Delta-associated tumors.
    關聯: International Journal of Molecular Sciences, v.15 n.7, pp.12442-12457
    Appears in Collections:[生物科技系(所)] 期刊論文

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