Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27909
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    標題: Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus
    作者: Yang, Ming-Hui
    Wang, Hsien-Yi
    Lu, Chi-Yu
    Tsai, Wan-Chi
    Lin, Po-Chiao
    Su, Shih-Bin
    Tyan, Yu-Chang
    貢獻者: 運動管理系
    關鍵字: Retinol-Binding-Protein
    Apolipoprotein A-Iv
    Human Pleural Effusion
    Alzheimers-Disease
    Mass-Spectrometry
    Vitamin-A
    Type-2
    Identification
    Serum
    Fluid
    日期: 2013
    上傳時間: 2014-05-26 10:48:36 (UTC+8)
    出版者: Hindawi Publishing Corporation
    摘要: Peritoneal dialysis (PD) is an increasingly accepted modality of renal replacement therapy. It provides the advantages of having a flexible lifestyle, stable hemodynamics, and better preservation of residual renal function. To enhance our understanding of the peritoneal dialysate of diabetesmellitus (DM), peritoneal dialysate proteins were identified by two-dimensional gel electrophoresis (2DE) combined with reverse-phase nano-ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (RP-nano-UPLC-ESI-MS/MS) followed by peptide fragmentation patterning. To validate the differential proteins, ELISA and Western blotting analyses were applied to detect candidate proteins that may be related to DM. We performed 2DE on the peritoneal dialysate samples, with detection of more than 300 spots. From this, 13 spots were excised, in-gel digested, and identified by RP-nano-UPLC-ESI-MS/MS. Ten of these showed significant differential expression between the DM and chronic glomerulonephritis (CGN) peritoneal dialysate samples. In this study, we conducted a comparative proteomic study on these two groups of dialysate that may provide evidence for understanding the different peritoneal protein changes. These proteins may not be new biomarkers; however, they may indicate a situation for possible drug treatment and can be the predictors of peritonitis for a validation study in the future.
    關聯: Biomed Research International,Vol.2013, 642964
    显示于类别:[運動管理系] 期刊論文

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