English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16812/19099 (88%)
Visitors : 6358026      Online Users : 311
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27909


    標題: Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus
    作者: Yang, Ming-Hui
    Wang, Hsien-Yi
    Lu, Chi-Yu
    Tsai, Wan-Chi
    Lin, Po-Chiao
    Su, Shih-Bin
    Tyan, Yu-Chang
    貢獻者: 運動管理系
    關鍵字: Retinol-Binding-Protein
    Apolipoprotein A-Iv
    Human Pleural Effusion
    Alzheimers-Disease
    Mass-Spectrometry
    Vitamin-A
    Type-2
    Identification
    Serum
    Fluid
    日期: 2013
    上傳時間: 2014-05-26 10:48:36 (UTC+8)
    出版者: Hindawi Publishing Corporation
    摘要: Peritoneal dialysis (PD) is an increasingly accepted modality of renal replacement therapy. It provides the advantages of having a flexible lifestyle, stable hemodynamics, and better preservation of residual renal function. To enhance our understanding of the peritoneal dialysate of diabetesmellitus (DM), peritoneal dialysate proteins were identified by two-dimensional gel electrophoresis (2DE) combined with reverse-phase nano-ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (RP-nano-UPLC-ESI-MS/MS) followed by peptide fragmentation patterning. To validate the differential proteins, ELISA and Western blotting analyses were applied to detect candidate proteins that may be related to DM. We performed 2DE on the peritoneal dialysate samples, with detection of more than 300 spots. From this, 13 spots were excised, in-gel digested, and identified by RP-nano-UPLC-ESI-MS/MS. Ten of these showed significant differential expression between the DM and chronic glomerulonephritis (CGN) peritoneal dialysate samples. In this study, we conducted a comparative proteomic study on these two groups of dialysate that may provide evidence for understanding the different peritoneal protein changes. These proteins may not be new biomarkers; however, they may indicate a situation for possible drug treatment and can be the predictors of peritonitis for a validation study in the future.
    關聯: Biomed Research International,Vol.2013, 642964
    Appears in Collections:[運動管理系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    310902800_27909.pdf705KbAdobe PDF492View/Open
    index.html0KbHTML498View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback