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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27823


    標題: Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
    作者: Wu, Chun-Chi
    Chen, Tzu-Hsiu
    Liu, Bing-Lan
    Wu, Li-Chen
    Chen, Yung-Ching
    Tzeng, Yew-Min
    Hsu, Shih-Lan
    貢獻者: 保健營養系
    醫藥化學系
    關鍵字: Dna-Damage Response
    Antitumor-Activity
    Colorectal-Cancer
    Natural-Products
    Protein Family
    Cytochrome-C
    Cyclodepsipeptides
    Gefitinib
    Death
    Adenocarcinoma
    日期: 2013
    上傳時間: 2014-05-26 10:45:07 (UTC+8)
    出版者: Hindawi Publishing Corporation
    摘要: Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. In this study, destruxin B was extracted and purified by ion-exchange chromatography, silica gel chromatography, and semipreparative high-performance liquid chromatography. The potential anticancer effects and molecular mechanisms of destruxin B in human nonsmall cell lung cancer cell lines were characterized. Our results showed that destruxin B induced apoptotic cell death in A549 cells. This event was accompanied by the activation of caspase-2, -3, and -9. Moreover, destruxin B increased the expression level of proapoptotic molecule, PUMA, while decreased antiapoptotic molecule Mcl-1. Additionally, the translocation of Bax from cytosol to mitochondrial membrane was observed upon destruxin B treatment. Knockdown of Bax by shRNA effectively attenuated destruxin-B-triggered apoptosis in A549 cells. Interestingly, similar toxic effects and underlying mechanisms including caspase activation, upregulation of PUMA, and downregulation of Mcl-1 were also observed in a p53-null lung cancer H1299 cell line upon destruxin B treatment. Taken together, our findings suggest that destruxin-B-induced apoptosis in human nonsmall cell lung cancer cells is via a Bcl-2 family-dependent mitochondrial pathway.
    Appears in Collections:[保健營養系(所) ] 期刊論文
    [醫藥化學系 ] 期刊論文

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