Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients

doi:10.1155/2013/863860

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標題:	Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients
作者:	Wu, Hsin-Yi Liao, Alex Chien Hwa Huang, Chien-Cheng Liao, Pao-Chi Chien, Chih-Chiang Kan, Wei-Chih Wang, Hsien-Yi
貢獻者:	運動管理系
關鍵字:	Histidine-Rich Glycoprotein Amyloid-P Component Capd Patients Protein Immune Fluid Expression Plasma Mice Identification
日期:	2013
上傳時間:	2014-05-26 10:44:18 (UTC+8)
出版者:	Hindawi Publishing Corporation
摘要:	Peritoneal dialysis (PD) frequently contributes to peritoneal damage which cannot be easily identified without invasive techniques, implying the urgent need for biomarkers and revealing mechanisms. Chronic glomerulonephritis (CGN) is one of the leading causes of receiving dialysis treatment. Here, we attempted to analyze the peritoneal dialysate collected from CGN patients when they receive continuous ambulatory peritoneal dialysis (CAPD) treatment for the first time and after a year to reveal the protein changes that resulted from PD. Proteins were displayed by two-dimensional gel electrophoresis (2DE). Altered gel spots were digested followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. Eight proteins were found to have differential expression levels between two groups. Their differential expressions were validated by Western blots in other sets of peritoneal dialysates. Proteins identified with higher levels in the first-time dialysate suggested their dominant appearance in CGN patients, while those that showed higher levels in peritoneal dialysate collected after one year may result from initial peritoneal inflammation or changes in the permeability of the peritoneum to middle-sized proteins. All the identified proteins may provide a perceptiveness of peritoneal changes caused by PD and may function as potential biomarkers or drug targets.
關聯:	Biomed Research International, v.2013 Article ID 863860
显示于类别:	[運動管理系] 期刊論文

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