New Cationic Biodegradable Poly(Urethane-co-Ester): Synthesis, Structural Characterization, Modification and Gene Delivery

doi:10.1163/092050610X541935

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/27730

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27730

Title:	New Cationic Biodegradable Poly(Urethane-co-Ester): Synthesis, Structural Characterization, Modification and Gene Delivery
Authors:	Chang, Wei-Yang Hsiao, Yu-Che Shau, Min-Da
Contributors:	生物科技系
Keywords:	Poly(Urethane-Co-Ester) Binary Mixture Blending Method Buffering Capacity Transfection Efficiency
Date:	2012
Issue Date:	2014-03-24 15:23:03 (UTC+8)
Publisher:	Vsp Bv
Abstract:	To improve the transfection efficiency of poly(urethane-co-ester) and the cytotoxicity of PEI25k with DNA, we synthesized a new poly(urethane-co-ester), PUE, bearing ester linkages and amino groups in the backbone and urethane linkages in the side-chain, and then prepared a binary mixture, PUE-PEI25k, using a physical blending method. The structure of PUE was confirmed by FT-IR and NMR spectra. Both poly(urethane-co-ester), PUE, and binary mixture PUE-PEI25k, readily self-assembled with plasmid DNA (pCMV-beta gal) in a HEPES buffer, were characterized by dynamic light scattering. The results revealed that PUE and PUE-PEI25k were able to self-assemble plasmid DNA into PUE/DNA and PUE-PEI25k/DNA nano-complexes small enough to enter a cell through endocytosis. Titration studies were performed to determine the buffering capacities of PUE and PUE-PEI25k. The COS-7 cell viability in the presence of PEI25k, PUE and PUE-PEI25k was studied. At low mass ratio of PUE/PEI25k (150:1), it was found that the PUE-PEI25k/DNA complexes were able to transfect COS-7 cells in vitro with a high efficiency comparable to a well-known gene carrier, PEI25k/DNA. The results indicate that the binary mixture PUE-PEI25k is an attractive cationic carrier for gene delivery and an interesting candidate for further study. (C) Koninklijke Brill NV, Leiden, 2012
Relation:	Journal of Biomaterials Science-Polymer Edition, 23(1-4), 27-41
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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