English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16812/19099 (88%)
Visitors : 6058757      Online Users : 42
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27710


    標題: IN VITRO AND IN VIVO THERAPEUTICS OF beta-THUJAPLICIN ON LPS-INDUCED INFLAMMATION IN MACROPHAGES AND SEPTIC SHOCK IN MICE
    作者: Shih, M-F.
    Chen, L-Y.
    Tsai, P-J.
    Cherng, J-Y.
    貢獻者: 藥學系
    關鍵字: Inflammation
    Beta-Thujaplicin
    Inos
    Tnf-Alpha
    Septic Shock
    日期: 2012-01
    上傳時間: 2014-03-21 16:18:25 (UTC+8)
    出版者: Biolife Sas
    摘要: beta-thujaplicin, an active constituent from Chamaecyparis obtusa, has been shown to have acaricidal and antimicrobial effects. Very few studies have focused on the potential of the anti-inflammatory effect of beta-thujaplicin. Moreover, its capability of inhibiting inflammatory mediators e.g. TNF-alpha gene transcription, nitric oxide (NO) and prostaglandin E2, remains unknown. Besides those molecular mechanisms behind the anti-inflammatory effect of beta-thujaplicin, solid proof of its effectiveness in vivo has not yet been studied. In our study, in vitro effects of beta-thujaplicin were verified on RAW 264.7 macrophages which were stimulated by LPS. Indomethacin was used as a positive control. The inducible NO production after stimulation was measured by Griess reagent. PGE2, IL-6 and TNF-alpha were measured by ELISA methods. Protein expressions of iNOS, COX2, and NF-kappa B were evaluated by Western blotting. Septic ICR mice were administered 20 mg/kg of LPS and then the mortality rate was monitored. Within the concentration range which was devoid of cytotoxicty, beta-thujaplicin exhibited a clear dose-dependent inhibition on LPS-induced NO production. Furthermore, beta-thujaplicin inhibited LPS-induced PGE2, IL-6, and TNF-alpha production as well as iNOS, COX2, and NF-kappa B protein expression more substantially potent than indomethacin. In agreement with the in vitro study, beta-thujaplicin was shown to be effective in vivo for inhibiting LPS-induced NO and TNF-alpha production and a significant decrease in mortality rate of mice suffering from septic shock was observed. This study demonstrates the potential of beta-thujaplicin in treatment of inflammation and sepsis. These effects occur through an efficient blockage of TNF-alpha and iNOS production. beta-thujaplicin efficacy is comparable to that of indomethacin thus it can be a substitution but bear less depletion of PGE2, making this compound very promising in clinical applications.
    關聯: International Journal of Immunopathology And Pharmacology v.25 n.1 pp.39-48
    Appears in Collections:[藥學系(所)] 期刊論文

    Files in This Item:

    There are no files associated with this item.



    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback