Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa

doi:10.3390/ijms13055598

CNU IR > College of Sustainable Environment and Recreation > Dept. of Environmental Resources Management > Periodical Articles > Item 310902800/27653

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27653

Title:	Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa
Authors:	Cheng, Hui-Ling Lee, Yan-Horn Hsiung, Dai-Shion Tseng, Mei-Chen
Contributors:	環境資源管理系
Keywords:	Catadromous Crab Cross-Specific Amplification Polymorphic Loci
Date:	2012-05
Issue Date:	2014-03-21 16:16:34 (UTC+8)
Publisher:	Mdpi Ag
Abstract:	The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. formosa population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the genetic diversity of P. formosa. With the exception of the Pfo15 locus, all of the remaining loci were polymorphic with allelic numbers ranging from 3-14. Heterozygosity within all 17 polymorphic loci ranged from 0.2-0.95 with an average of 0.55, which suggested that these loci are proper markers for studying population genetics. After we tested cross-specific amplification, eight and six primer sets could be successfully used for the amplification of microsatellite loci in morphologically similar Eriocheir sinensis and E. japonica, respectively; this suggests that they are useful markers for closely related species.
Relation:	International Journal of Molecular Sciences, 13(5), 5598-5606
Appears in Collections:	[Dept. of Environmental Resources Management] Periodical Articles

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