FERRITIN-RED FLUORESCENT PROTEIN FUSION REPORTER FOR MAGNETIC RESONANCE AND OPTICAL IMAGING

doi:10.4015/S1016237212500287

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/27594

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標題:	FERRITIN-RED FLUORESCENT PROTEIN FUSION REPORTER FOR MAGNETIC RESONANCE AND OPTICAL IMAGING
作者:	Liu, Shan-Wen Cho, Kuan-Hung Chen, Mei-Ru Yu, Hsiao-Chi Kao, Yu-Ying Tsou, Tsui-Chun Lin, Ching-Po Chen, Chin-Tu Hsu, Ching-Han Lin, Kurt Ming-Chao Lin, Kurt Ming-Chao � Tsou, Tsui-Chun�
貢獻者:	生物科技系
關鍵字:	Magnetic Resonance Imaging Multimodality Imaging Red Fluorescent Protein Adenovirus Iron Loading
日期:	2012-08
上傳時間:	2014-03-21 16:14:39 (UTC+8)
出版者:	World Scientific Publ Co Pte Ltd
摘要:	In this report, we generated a ferritin and red fluorescent protein fusion reporter gene that enables the visualization of transgene expression in living animals by magnetic resonance imaging (MRI) and optical imaging. Ferritin heavy chain (FTH) or light chain (FTL) was linked to the N terminus of the monomeric DsRed red fluorescent protein to create FTH-DsRed and FTL-DsRed, MRI-fluorescence dual reporters. Transfection of these dual reporters into cells resulted in increased iron loading and strong red fluorescence in cells. Adenoviral vectors to express FTH-DsRed or FTL-DsRed fusion reporter in infected cells were created, but only the adenovirus expressing FTH-DsRed resulted in a high level of red fluorescence in cells. Delivery and expression of FTH-DsRed in the mouse brain using adenovirus was detected by MRI and fluorescence imaging, revealing a T-2 shortening effect and an increase of contrast in T-2-weighted images at the sites co-localized with strong red fluorescence. While the details of the structure of ferritin-DsRed fusion reporter remains to be solved, this dual reporter is useful for visualizing dynamic processes such as the migration of reporter-transfected stem cells or metastasis of tumors using MRI with the added flexibility of combining optical tools such as fluorescence activated cell sorting and fluorescence microscopy.
關聯:	Biomedical Engineering-Applications Basis Communications, 24(4), 333-341
顯示於類別:	[生物科技系(所)] 期刊論文

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