Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27594
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16999/19305 (88%)
Visitors : 9752427      Online Users : 729
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27594


    Title: FERRITIN-RED FLUORESCENT PROTEIN FUSION REPORTER FOR MAGNETIC RESONANCE AND OPTICAL IMAGING
    Authors: Liu, Shan-Wen
    Cho, Kuan-Hung
    Chen, Mei-Ru
    Yu, Hsiao-Chi
    Kao, Yu-Ying
    Tsou, Tsui-Chun
    Lin, Ching-Po
    Chen, Chin-Tu
    Hsu, Ching-Han
    Lin, Kurt Ming-Chao
    Lin, Kurt Ming-Chao �
    Tsou, Tsui-Chun�
    Contributors: 生物科技系
    Keywords: Magnetic Resonance Imaging
    Multimodality Imaging
    Red Fluorescent Protein
    Adenovirus
    Iron Loading
    Date: 2012-08
    Issue Date: 2014-03-21 16:14:39 (UTC+8)
    Publisher: World Scientific Publ Co Pte Ltd
    Abstract: In this report, we generated a ferritin and red fluorescent protein fusion reporter gene that enables the visualization of transgene expression in living animals by magnetic resonance imaging (MRI) and optical imaging. Ferritin heavy chain (FTH) or light chain (FTL) was linked to the N terminus of the monomeric DsRed red fluorescent protein to create FTH-DsRed and FTL-DsRed, MRI-fluorescence dual reporters. Transfection of these dual reporters into cells resulted in increased iron loading and strong red fluorescence in cells. Adenoviral vectors to express FTH-DsRed or FTL-DsRed fusion reporter in infected cells were created, but only the adenovirus expressing FTH-DsRed resulted in a high level of red fluorescence in cells. Delivery and expression of FTH-DsRed in the mouse brain using adenovirus was detected by MRI and fluorescence imaging, revealing a T-2 shortening effect and an increase of contrast in T-2-weighted images at the sites co-localized with strong red fluorescence. While the details of the structure of ferritin-DsRed fusion reporter remains to be solved, this dual reporter is useful for visualizing dynamic processes such as the migration of reporter-transfected stem cells or metastasis of tumors using MRI with the added flexibility of combining optical tools such as fluorescence activated cell sorting and fluorescence microscopy.
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML299View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback