trans-Caffeic Acid Stearyl Ester from Paeonia suffruticosa Inhibits Melanin Synthesis by cAMP-Mediating Down-Regulation of alpha-Melanocyte-Stimulating Hormone-Stimulated Melanogenesis Signaling Pathway in B16 Cells

doi:10.1248/bpb.b12-00619

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/27517

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27517

Title:	trans-Caffeic Acid Stearyl Ester from Paeonia suffruticosa Inhibits Melanin Synthesis by cAMP-Mediating Down-Regulation of alpha-Melanocyte-Stimulating Hormone-Stimulated Melanogenesis Signaling Pathway in B16 Cells
Authors:	Liang, Chia-Hua Chou, Tzung-Han Tseng, Ya-Ping Ding, Hsiou-Yu
Contributors:	化妝品應用管理系
Keywords:	Trans-Caffeic Acid Stearyl Ester Paeonia Suffruticosa Melanogenesis Tyrosinase
Date:	2012-12
Issue Date:	2014-03-21 16:12:07 (UTC+8)
Publisher:	Pharmaceutical Soc Japan
Abstract:	trans-Caffeic acid stearyl ester (TCASE) from the root cortex of Paeonia suffruticosa ANDREWS is a traditional medicinal herb that has several beneficial properties. However, the inhibitory effect of TCASE on melanogenesis has not been explored. In the cell viability assay, TCASE did not show a cytotoxic effect at a dose of 65 mu m for 48h in B16, HaCaT and Hs68 cells. TCASE considerably inhibits melanin synthesis, and reduces intracellular cyclic adenosine monophosphate (cAMP) levels, tyrosinase activity and L-3-(3,4-dihydroxyphenyl)-alanine (DOPA) oxidase activity in a concentration-dependent manner in the presence of alpha-melanocyte-stimulating hormone (alpha-MSH) in B16 cells, and the inhibition efficiency of TCASE exceeds that of ascorbic acid and arbutin. TCASE reduces melanocortin-1 receptor (MC1R), microphthalmia transcription factor (MITF), tyrosinase, tyrosinase-related protein-2 (TRP-2) and TRP-1 mRNA and protein levels in B16 cells. Based on the findings, TCASE is posited to inhibit melanogenesis signaling while suppressing cAMP levels and, subsequently, MC1R, MITE, tyrosinase, TRP-2 and TRP-1 down-regulation, resulting in the suppression of tyrosinase activity, DOPA oxidase activity and melanin synthesis.
Relation:	Biological & Pharmaceutical Bulletin v.35 n.12 pp.2198-2203
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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