Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27500
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 18270/20497 (89%)
造访人次 : 11791965      在线人数 : 1007
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27500


    標題: 百合之組織培養(Ⅲ).花藥培養
    Studies on the Tissue Cultiure of Lilium longiflorum Thunb. (Ⅲ). Anther Culture
    作者: 周哲彥
    貢獻者: 私立嘉南藥學專科學校
    日期: 1979
    上傳時間: 2014-03-17 16:15:23 (UTC+8)
    摘要: 採不同長度的鐵炮百合( Lilium longiflorum)之花蕾( Floral bud )經鏡檢其花藥發育期再配合花蕾之長度而區分成: (一)花粉母細胞期( 1-2 cm ),(二)二分子或四分子至單核花粉期( 2-3 cm ),(三)雙核花粉期( 3cm以上)。各期花蕾在無菌室經消毒處理後切取其花藥置於改良之Murashige and Skoog培養基上,在溫度27 ± 2℃及十二小時光期(約3000 lux )的環境下培養,經一星期後,各期花藥皆有膨脹扭轉現象,缺少NAA之培養基其花藥不能繼續生長,在含有NAA和Kine-tin的培養基之各期花藥均能繼續生長,花藥色澤也由淡黃或淺綠轉化成綠色,約二十天後部分花藥可由裂口或由其中軸花絲切口處誘出癒合組織,並長出根和幼苗,在含1ppm Kinetin和 l ppm NAA培養基上的四分子期至單核期花藥較易形成癒合組織。利用醋酸洋紅塗片鏡檢癒合組織之染色體數,顯示多數癒合組織衍自雙倍體之體細胞。
    Different development stages of anthers were aseptically removed from flower buds of Lilium longiflorum And inoculated on a modified Murashige and Skoog medium. There were incubated at 27 ± 2 ℃ under illumination with 3000 lux fluorescent light for 12 hr daily. The medium containing NAA and kinetin promoted the anther growth. After one week incubation the anther swelled, twisted, and turned green.Another three weeks later,callus proliferated from wound tissue of the some anthers and differentiat-ed into shoots and roots. The microscopic examination of callus chromo-some showed that the callus consisted of diploid cells.
    關聯: 嘉南學報 5期 : p.47-52
    Appears in Collections:[嘉南學報] 5期 (1979)

    Files in This Item:

    There are no files associated with this item.



    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback