Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27498
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18034/20233 (89%)
造訪人次 : 23369544      線上人數 : 497
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/27498


    標題: Rapid Determination of Triamcinolone in Tablets and the Mechanism of Solvent Effect in Zaffaroni Reaction
    Triamcinolone 錠劑之快速定量法及Zaffaroni反應中溶媒的效應機序
    作者: Lee, Tsu-Chi
    Wang, Chao-Hsiung
    Hsu, Hsingchu
    貢獻者: 私立嘉南藥學專科學校
    行政院衛生署藥政處
    日期: 1979
    上傳時間: 2014-03-17 16:15:17 (UTC+8)
    摘要: A rapid assay procedure for triamcinolone in tablets was described which enabled a sensitive determination of triamcinolone in tablets in less than one fifth time-consuming than official USP method to give identical result. Triamcinolone in tablets was dissolved and extracted with N,N-dimethylformamide rapidly followed by methanol and chloroform dilution tO 5 mcg per ml. The colorimetric determination after Zaffar-oni reaction of α-ketol side chain was carried out at triamcinolone con-centration 3 mcg per ml. The mechanism of solvent effect in Zaffaroni reaction was also discussed. It was revealed that the rate of reaction was accelerated by solvents of low interna1 pressure.In practical sense,the addition of chloroform or ethyl ether in the reaction media would facilitate instant color production at room temperature due to the redu-ction of the solubility parameter difference between Solvent and the activated complex. The method was applicable to most of water insoluble α-ketol corticoids in solid preparations and was valuable in in-process and off-process uniformjty control.
    USP及BPC皆使用乙醇為溶媒,利用機械振盪二小時抽出錠劑粉未內之Triamcinolone.USP更在標準液之泡製上完全使用氯仿。實際上, Triamcinolone在乙醇及氯仿中之溶解度極小,且溶解速度極慢,不但操作費時,且易因抽出程度之差異,難獲得穩定之定量結果。日本藥局方並不收載Triamcinolone錠劑,但對原料之定量採用甲醇為溶媒,雖比乙醇及氯仿為佳,但未能顯著地縮短操作時間,而且日本藥局方使用UV吸光度之測定,靈敏度遠較USP及BPC之呈色法為低。像Tria-mcinolone一類製劑,因劑量微少,須嚴格要求均勻度,必須在生產過程中加以經常性的檢定。為建立快速,靈敏之定量方法,以利生產中之品管須要,本研究檢討Triamcinolone在各種溶媒中之溶解度及溶解速度,發現N,N-dimethylformamide及N,N-d imethylacetamide為Triamcinolone最優異之溶媒。利用N,N-d imethylformamide溶出Triamcinolone經甲醇及氯仿之稀釋,在鹹性液中和Tetrazolium blue進行Zaffaroni反應呈色,則Triamcinolone可在 3 mcg/ml之低濃度下被正確之定量。氯仿之存在對Zaffaroni呈色反應極為重要,若不添加氯仿於測定液中,則在室溫下,不能呈色。經檢討Zaffaroni反應中各種溶媒的效應,發現降低反應液之內壓( internal pressure)能加速Zaffaroni反應。在實用上, Chloroform,及Ethyl ether皆可應用於反應系中,扮演即刻呈色作用劑,縮短操作時間。使用N,N-dimethylformamide之快速溶出法所得之溶液,不適於利用UV吸光度測定法,因為此溶媒在238 nm處,有吸光性會干擾測定結果,造成誤差。但若使用Zaffaroni呈色法則可獲得和USP法定方法同樣正確之結果而且快速五倍以上。本法可通用於非水溶α-ketol corticoids固體製劑之定量。
    關聯: 嘉南學報 5期 : p.31-38
    顯示於類別:[藥學系(所)] 期刊論文
    [嘉南學報] 5期 (1979)

    文件中的檔案:

    沒有與此文件相關的檔案.



    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋