七種中草藥水萃物之生物活性研究與探討

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標題:	七種中草藥水萃物之生物活性研究與探討 Evaluation of the Bioactivities of Aqueous Extract From Seven Herbal Plants
作者:	楊哲彰
貢獻者:	藥學系呂昆霖
關鍵字:	中草藥抗氧化抗菌抗發炎粉紫薇 Chinese herbal medicine antioxidation anti-inflammation antibacterial activity Lagerstroemia indica L.
日期:	2013
上傳時間:	2014-03-11 16:27:57 (UTC+8)
摘要:	中草藥植物在亞洲用於治療疾病上已行之有年，但卻缺乏科學性實驗數據來證實其活性強弱。本研究之目的著重於中草藥水萃物之生物活性探討，比較是否同時具有顯著之抗氧化、抗菌與抗發炎效果。實驗結果顯示，在抗菌方面，由紙錠擴散法結果得知，粉紫薇(Lagerstroemia indica L.)對於大腸桿菌、綠膿桿菌、金黃色葡萄球菌等皆有顯著之抑制效果，抑制圈大小分別為19.17±1.61、18.50±1.41、14.33±0.76；三椏苦(Evodia Lepta(Spreng.)Meer.)對於大腸桿菌、金黃色葡萄球菌等皆有顯著之抑制效果，抑制圈大小分別為10.33±2.08、15.67±0.58 mm；由最小抑菌濃度與最小殺菌濃度結果得知，所有樣品在濃度小於1 mg/mL以下均無明顯之抗菌活性。在抗氧化方面，七種中草藥水萃物中，總類黃酮含量最高者為大丁癀(Euonymus laxiflorus Champ.)、三椏苦(Evodia Lepta(Spreng.)Meer.)，分別為158.40±2.26、155.60±3.59 mg CE/g extract；總酚含量最高者為三椏苦(Evodia Lepta(Spreng.)Meer.)、金露花(Duranta repens L.)，分別為293.70±16.4、212.90±5.14 mg GAE/g extract；在DPPH自由基清除率效果最佳者為粉紫薇(Lagerstroemia indica L.)、三椏苦(Evodia Lepta(Spreng.)Meer.)，EC50分別為16.09±0.85、29.14±1.91 μg/mL；還原力效果最佳者為粉紫薇(Lagerstroemia indica L.)、三椏苦(Evodia Lepta(Spreng.)Meer.)，EC50分別為10.92±0.45、19.75±0.97 μg/mL；總抗氧化能力效果最佳者為粉紫薇(Lagerstroemia indica L.)、三椏苦(Evodia Lepta(Spreng.)Meer.)，分別為2.64±0.17、2.46±0.50 mmol TE/g extract；螯合亞鐵離子效果最佳者為粉紫薇(Lagerstroemia indica L.)、草荳蔻(Alpina katsumadai Hayata)，EC50分別為1.250±0.10、1.610±0.05 mg/mL；脂質過氧化抑制效果最佳者為羊蹄甲(Bauhinia variegata L.)、草荳蔻(Alpina katsumadai Hayata)，抑制率分別為85.02±4.61、83.91±1.73%。在抗發炎方面，粉紫薇(Lagerstroemia indica L.)、草荳蔻(Alpina katsumadai Hayata)、大丁癀(Euonymus laxiflorus Champ.)均有顯著之抑制效果。在細胞存活率結果得知，所有樣品在濃度200 μg/mL以下，細胞存活率均在80%以上，無細胞毒性。綜合以上結果，顯示粉紫薇(Lagerstroemia indica L.)具有顯著抗氧化，抗發炎與抗菌活性，未來期待能加以推廣應用於臨床治療、藥品、化粧品、生技產業等領域中。 Medicinal herbs have used for a very long time in human’s history, but lack of the significant evidence and data in science. In the present investigation, we evaluated whether those seven herbal aqueous extracts contained the antibacterial, antioxidative and anti-inflammatory capacity at the same time.Our results showed that Lagerstroemia indica L. exhibited a good antibacterial activity against B. subtilis and two Gram-negative bacteria (E. coli, P. aeruginosa) with the inhibitory zone of 14-19 mm. Lagerstroemia indica L. and Evodia Lepta(Spreng.)Meer. displayed the lowest value of EC50 (16.09±0.85 and 29.14±1.91 μg/mL) to scavenge the DPPH radicals. The total flavonoid content of Euonymus laxiflorus Champ. and Evodia Lepta(Spreng.)Meer. were equal to 158.40±2.26 and 155.60±3.59 mg CE/g extract, respectively. The total phenolic content of Evodia Lepta(Spreng.)Meer. and Duranta repens L. were up to 293.70±16.4 and 212.90±5.14 mg GAE/g extract, respectively. For the reducing power assay, the lowest EC50 value was shown in Lagerstroemia indica L. (10.92±0.45 μg/mL) and Evodia Lepta(Spreng.)Meer. (19.75±0.97 μg/mL). The TEAC value of Lagerstroemia indica L. and Evodia Lepta(Spreng.)Meer. were equal to 2.64±0.17 and 2.46±0.50 mmol TE/g extract, respectively. For the ferrous ion-chelating ability, the lowest EC50 value was exhibited by Lagerstroemia indica L. (1.250±0.10 mg/mL) and Alpina katsumadai Hayata (1.610±0.05 mg/mL). The best value of inhibiting lipid peroxidation were 85.02±4.61% and 83.91±1.73% in 100 μg/mL of Bauhinia variegata L. and Alpina katsumadai Hayata aqueous extract. For the anti-inflammation test, Lagerstroemia indica L., Alpina katsumadai Hayata and Euonymus laxiflorus Champ. could decrease the hyaluronidase activity. For the cell viability assay, all the samples showed no cytotoxicity below 200 μg/mL. Therefore, the multiple functions of Lagerstroemia indica L. will be applied in the clinical, medical, cosmetic and biotechnological fields.
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