Enzyme Immunoassay of Urokinase from Human Urine

CNU IR > Chna Nan Annual Bulletin > Vol.8 (1982) > Item 310902800/27051

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Title:	Enzyme Immunoassay of Urokinase from Human Urine 尿激酶之酵素免疫分析
Authors:	Yeh, Dong-Bor Lee, Ping-Do Su, Jong-Ching
Contributors:	食品衛生科
Date:	1982
Issue Date:	2013-10-25 09:22:21 (UTC+8)
Abstract:	In this study, a more specific enzyme-immunoassay method for the urokinase activity has been developed. The anti-UK (antibody) was isolated rom the rabbit serum and coupled with horseradish peroxidase as the marker enzyme. The enzyme conjugate thus prepared was used for the quantitative analyses of urokinase by a competitive immunoassay in the presence of immobilized UK (UK-Sepharose). This method was comparable in sensitivity to the traditional fibrinolytic assay and an established radio-immunoassay. 本研究敘述一種關於尿激酶的酵素免疫分析法，此法係利用過氧化酶標幟在經部份純化的尿激酶抗體上，再根據競爭性免疫反應以達到定量樣品中尿激酶濃度之目的。其靈敏度（最低檢出濃度為1國際單位/毫升）雖與傳統的纖維蛋白平板分析法或已開發的放射性免疫分析法相似，卻較一般的酯解或醯胺解分析法為高。
Relation:	嘉南學報 8, pp.1-10
Appears in Collections:	[Chna Nan Annual Bulletin] Vol.8 (1982) [Dept. of Food Science & Technology] Periodical Articles

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