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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27051


    標題: Enzyme Immunoassay of Urokinase from Human Urine
    尿激酶之酵素免疫分析
    作者: Yeh, Dong-Bor
    Lee, Ping-Do
    Su, Jong-Ching
    貢獻者: 食品衛生科
    日期: 1982
    上傳時間: 2013-10-25 09:22:21 (UTC+8)
    摘要: In this study, a more specific enzyme-immunoassay method for the urokinase activity has been developed. The anti-UK (antibody) was isolated rom the rabbit serum and coupled with horseradish peroxidase as the marker enzyme. The enzyme conjugate thus prepared was used for the quantitative analyses of urokinase by a competitive immunoassay in the presence of immobilized UK (UK-Sepharose). This method was comparable in sensitivity to the traditional fibrinolytic assay and an established radio-immunoassay.
    本研究敘述一種關於尿激酶的酵素免疫分析法,此法係利用過氧化酶標幟在經部份純化的尿激酶抗體上,再根據競爭性免疫反應以達到定量樣品中尿激酶濃度之目的。其靈敏度(最低檢出濃度為1國際單位/毫升)雖與傳統的纖維蛋白平板分析法或已開發的放射性免疫分析法相似,卻較一般的酯解或醯胺解分析法為高。
    關聯: 嘉南學報 8, pp.1-10
    Appears in Collections:[嘉南學報] 8 期 (1982)
    [ 食品科技系 ] 期刊論文

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