English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17330/19636 (88%)
Visitors : 4585076      Online Users : 356
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/26851

    標題: 臺灣蜂膠美白活性之探討
    The study of Taiwanese propolis on skin-whitening activity
    作者: 陳秋蘭
    貢獻者: 嘉南藥理科技大學藥學系
    關鍵字: 台灣蜂膠
    Taiwanese propolis
    日期: 2012-12
    上傳時間: 2013-08-14 10:42:39 (UTC+8)
    摘要: 蜂膠是一種由蜜蜂採集各種植物的嫩芽或樹皮之汁液,再與蜜蜂的唾液酵素、花粉、及蜂蠟等混合所得的膠狀黏性物質,用來塗裝在蜂巢的內壁,可用來防禦外敵、也可對抗微生物感染,避免食物的腐敗。蜂膠具有許多生理活性,包含有抗細菌、抗病毒、抗發炎及及抗腫瘤等。蜂膠中的主成份黃酮類也具有很好的抗氧化能力,可以對抗紫外線,避免皮膚受到傷害,因而被應用在化妝品和保養品上面。但當蜂膠產品塗抹在皮膚上,是否會進入皮膚角質層,而產生美白之效果?因此,本研究想先評估台灣蜂膠的乙醇萃取物及其分層是否能抑制黑色素之生合成。首先先看台灣蜂膠的乙醇萃取物及其分層是否會抑制酪胺酸酶的活性,再以MTT 分析其對B16 黑色素瘤細胞是否具細胞毒性,並以波長405nm 分析其在B16 細胞的黑色素含量。結果發現蜂膠的乙醇萃取物及其分層(BG-2 及BG-3)可抑制酪胺酸酶活性,但對B16 細胞具有細胞毒性,且呈劑量及時間相關性,此細胞毒殺作用可能是因誘導細胞凋亡所致,但詳細機制則需更進一步的研究分析。
    Propolis is a hive product that bees manufacture from balsamic resins actively secreted by plants on leaf buds and barks. It possesses several biological activities such as antibacterial, antiviral, antiinflammatory, and antitumor effects. Propolis and its main flavonoids components also show high antioxidant activity. Furthermore, these substances evidence effectiveness as broad spectrum UVB and UVA photoprotection sunscreens. The combination of these characteristics moves up propolis and its main flavonoids components to the class of cosmeceuticals, as possible active ingredient of sunscreen commercial formulations for their protective and preventive properties. However, when the epidermis is exposed to propolis products, the exact mode of melanogenesis is yet to be determined. Therefore, the objective of our study is to investigate the whitening and melanogenesis inhibitory potential of Taiwanese propolis ethanol extract and its fractions at tyrosinase activity assay and melanoma cells. Thus, we first screened the inhibit potential of propolis extract and its fractions on tyrosinase activity. We further determined the cytotoxicity of propolis extract and its fractions in mouse melanoma B16 cells by using tetrazolium salt (MTT). The effects of propolis extract and its fractions on melanin content in B16 cells were also measured by UV at OD405. The data showed that Taiwanese propolis extract and its fractions (BG-2 and BG-3) inhibited mushroom tyrosinase activity in vitro. They were cytotoxic to B16 cells in a dose-and time-dependent manner and this effect may be from apoptosis induction, but the mechanism is needed further study.
    關聯: 嘉南學報(科技類) 38期:p.82-90
    Appears in Collections:[嘉南學報] 38 期 (2012)
    [藥學系(所)] 期刊論文
    [醫藥化學系 ] 期刊論文

    Files in This Item:

    File SizeFormat
    38_82_90.pdf1274KbAdobe PDF4197View/Open

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback