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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/26836

    標題: 移動相中添加HP-β-環糊精在RPLC上分離二氯酚異構物
    Separation of Isomeric Dichlorophenols by RPLC Using HP-β-cyclodextrin as Mobile Phase Additives
    作者: 廖麗芳
    貢獻者: 輔英科技大學應用化學及材料科學系
    關鍵字: 逆相液相層析
    Reversed-Phase Liquid Chromatography (RPLC)
    日期: 2012-12
    上傳時間: 2013-08-14 10:42:22 (UTC+8)
    摘要: 本研究在移動相中添加HP-β-CD,利用RP-C18 靜相以分離6 種二氯酚異構物。結果顯示在移動相中,HP-β-環糊精的濃度及甲醇與水比例,會顯著影響二氯酚異構物的分離效果。經由層析圖得知,添加1.25 mM HP- β-環糊精於甲醇與水比例為50:50 的混合溶液當移動相,利用RP-C18 靜相管柱可以有效分離6種二氯酚異構物。從二氯酚異構物的滯留行為可知,包藏錯合物在分離機轉上扮演重要的角色。
    The isomeric dichlorophenols were separated by high-performance liquid chromatography using HP-β-cyclodextrin as mobile phase additives. Effects of HP-β-CD concentration and methanol content of mobile phase on the separation of isomeric dichlorophenols were investigated. Chromatogram of six isomeric dichlorophenols shows that the effective separations were achieved on a RPC18 stationary phase using 1.25mM HP-β-cyclodextrin as mobile phase additives. The chromatographic results indicate that inclusion complexes play a dominant role in the retention mechanism.
    Appears in Collections:[嘉南學報] 38 期 (2012)
    [化妝品應用與管理系(所)] 期刊論文

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