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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/26692

    標題: Genome-based deletion analysis reveals the prenyl xanthone biosynthesis pathway in Aspergillus nidulans.
    作者: James, F.Sanchez
    Entwistle, Ruth
    Hung, Jui-Hsiang
    Yaegashi, Junko
    Jain, Sofina
    Chiang, Yi-Ming
    Clay, C.C.Wang
    Berl, R.Oakley
    貢獻者: 生物科技系
    關鍵字: Secondary Metabolism
    Gene Clusters
    日期: 2011-05
    上傳時間: 2013-06-05 16:42:47 (UTC+8)
    出版者: ACS
    Amer Chemical Soc
    摘要: Xanthones are a class of molecules that bind to a number of drug targets and possess a myriad of biological properties. An understanding of xanthone biosynthesis at the genetic level should facilitate engineering of second-generation molecules and increasing production of first-generation compounds. The filamentous fungus Aspergillus nidulans has been found to produce two prenylated xanthones, shamixanthone and emericellin, and we report the discovery of two more, variecoxanthone A and epishamixanthone. Using targeted deletions that we created, we determined that a cluster of 10 genes including a polyketide synthase gene, mdpG, is required for prenyl xanthone biosynthesis. mdpG was shown to be required for the synthesis of the anthraquinone emodin, monodictyphenone, and related compounds, and our data indicate that emodin and monodictyphenone are precursors of prenyl xanthones. Isolation of intermediate compounds from the deletion strains provided valuable clues as to the biosynthetic pathway, but no genes accounting for the prenylations were located within the cluster. To find the genes responsible for prenylation, we identified and deleted seven putative prenyltransferases in the A. nidulans genome. We found that two prenyltransferase genes, distant from the cluster, were necessary for prenyl xanthone synthesis. These genes belong to the fungal indole prenyltransferase family that had previously been shown to be responsible for the prenylation of amino acid derivatives. In addition, another prenyl xanthone biosynthesis gene is proximal to one of the prenyltransferase genes. Our data, in aggregate, allow us to propose a complete biosynthetic pathway for the A. nidulans xanthones.
    關聯: Journal of the American Chemical Society 133(11), pp.4010-4017
    Appears in Collections:[生物科技系(所)] 期刊論文

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