Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/26690
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17109/19415 (88%)
Visitors : 2623598      Online Users : 288
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/26690


    Title: Citrus flavonoid, 5-demethylnobiletin, suppresses scavenger receptor expression in THP-1 cells and alters lipid homeostasis in HepG2 liver cells.
    Authors: Yen, Jui-Hung
    Weng, Ching-Yi
    Li, Shiming
    Lo, Ya-Hsuan
    Pan, Min-Hsiung
    Fu, Shih-Hang
    Ho, Chi-Tang
    Wu2, Ming-Jiuan
    Contributors: 生物科技系
    Keywords: Acyl CoA:diacylglycerol acyltransferase 2
    Atherogenesis
    CD36
    c-Jun NH2-terminal kinase
    Steroid-response element-binding protein-2
    Date: 2011-05
    Issue Date: 2013-06-05 16:42:46 (UTC+8)
    Publisher: Wiley
    Wiley-Blackwell
    Abstract: Scope: Nobiletin, a polymethoxyflavone from the peel of citrus fruits, has been reported to inhibit modified LDL uptake in macrophages and enhance hepatic LDL receptor expression and activity. We report the anti-atherogenic effect and mechanism of 5-demethylnobiletin, an auto-hydrolysis product of nobiletin.

    Methods and results: 5-Demethylnobiletin significantly attenuated phorbol 12-myristate 13-acetate-induced gene expression and activity of scavenger receptors, CD36, scavenger receptor-A and lectin-like oxidized LDL receptor-1. The inhibitory effect is partly associated with the inhibition of protein-kinase C activity and c-Jun NH2-terminal kinase 1/2 phosphorylation, thereby inhibiting the activation of activator protein-1 and nuclear factor-κB. 5-Demethylnobiletin treatment also led to reduction of oxidized LDL-induced CD36 mRNA expression and blockade of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanide perchlorate-modified LDL uptake in THP-1-derived macrophages. In the human hepatoma cell line HepG2, 5-demethylnobiletin significantly induced LDL receptor activity and transcription, at least in part, through steroid-response element-binding protein-2 activation. 5-Demethylnobiletin also decreased the mRNA expression of acyl CoA:diacylglycerol acyltransferase 2, the key enzyme involved in the hepatic triacylglycerol biosyntheses.

    Conclusion: Current results suggest that 5-demethylnobiletin has diverse anti-atherogenic bioactivities. It is more potent in inhibiting monocyte-to-macrophage differentiation and foam cell formation than its permethoxylated counterpart, nobiletin. It exhibits similar hypolipidemic activity as nobiletin and both can enhance LDL receptor gene expression and activity and decreased acyl CoA:diacylglycerol acyltransferase 2 expression.
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML670View/Open
    PDF0KbHTML526View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback