三種不同的磷脂質，分別名為雙油酸磷脂醯膽鹼 ( DOPC ) 、雙硬脂酸磷脂醯膽鹼 ( DSPC ) 及雙棕梠酸磷脂醯膽鹼 ( DPPC ) ，被用來與油體鈣蛋白製備人造油體。使用DSPC與DPPC皆能製備出與使用DOPC安定性相當的人造油體。 Native seed oil bodies are lipid storage organelles and comprise an oil matrix shielded by a monolayer of phospholipid (PL) and specialized proteins. Artificial oil bodies (AOBs) can be reconstituted with oil, PL, and one of the proteins like caleosin. AOBs have been used as an oral drug delivery system.
Caleosin is a unique calcium binding protein anchoring to the surface of seed oil bodies by its central hydrophobic domain. Its property is changed when the amphiphatic α-helix or proline-knot subdomain in the hydrophobic domain of caleosin is truncated.
In this study, the potential of caleosin and its modified forms as an emulsifier of emulsions, as a stabilizer of liposomes and the effect of other kinds of PL on the stability of AOBs were studied. The turbidity test was used as a tool to evaluate the stability of emulsions, liposomes or AOBs. Besides, particle size and surface potential were also used to evaluate the stability of liposomes.
It was found that caleosin can be used an emulsifier to form oil in water emulsions. The emulsions made by caleosin were more stable than those made by modified caleosins.
Caleosin and Del-H were found tobe used in the preparation of liposomes. The analysis of particle size and surface potential shows that they can decrease the particle size and increase surface potential of liposomes.
Three kinds of PL, namely, dioleoyl phosphatidylcholine ( DOPC ), distearoyl phosphatidylcholine (DSPC), or distearoyl phosphatidylcholine (DPPC) were used to prepare AOBs with caleosin. AOBs made from DSPC and DPPC were found as stable as those made from DOPC.