|摘要: ||本研究以由化合物Y、T、H進行抗氧化效能評估及細胞毒殺作用活性篩選， 化合物Y、T、H均有良好的清除DPPH•自由基能力以及還原力效果，而在細胞內活性氧試驗中，經H2O2 刺激後， 化合物Y、T、H能抑制活性氧生成並增加榖胱甘肽生成量以及抗氧化酵素catalase和GPx活性增加。並藉由UV照射後誘導人類角質株化細胞(HaCaT)調控影響發炎相關因子mitogen-activated protein kinase (MAPK)家族與轉錄因子NF-κB，進而達到抗氧化性壓力及降低發炎反應，達到預防皮膚細胞癌化之功效。
In our preliminary examination, the compounds were evaluated antioxidant activities and interacted individually with serial human cancer cells, results that antioxidant activities of compounds Y, T, H were evaluated by measuring DPPH free-radical scavenging activities and reducing power. Determination the reactive oxygen species (ROS) content and reduced glutathione (GSH) formation in H2O2-treated HaCaT cells by compounds Y, T, H. And regulation the mitogen-activated protein kinase (MAPK) family and the transcription factor NF-κB in UV irradiation human keratinocytes (HaCaT cells) by compounds Y, T, H. Thus compounds Y, T, H can achieve resistance to oxidation stress and reduce inflammation, to prevent the effect of skin cell carcinoma.
The cytotoxicity results show that compounds Y, T, H expressed less toxic to normal skin cells than skin cancer cells, suggesting that compounds Y, T, H may have potential to be developed effective drugs for skin cancer cells without damaging skin normal cells. After treatment with compounds Y, T, H in BCC cells, cell cycle arrested in S-G2/M phase with a markedly increased apoptotic sub-G1 peak, mitochondria membrane potential (MMP) reduced, the expression of p53, Caspase revealed a more significant increased than the untreated control. Reduce metastasis and invasion of melanoma (A375). Expected compounds Y, T, H have potential for an effective and specific drug to skin cancer cells, can minimize the damage to normal cell and provide a better therapeutic method to skin carcinoma.