Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/25230
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 16886/19260 (88%)
造访人次 : 7536426      在线人数 : 776
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cnu.edu.tw/handle/310902800/25230


    標題: Site-Saturation Mutagenesis of Leucine 134 of Bacillus licheniformis Nucleotide Exchange Factor GrpE Reveals the Importance of this Residue to the Co-chaperone Activity
    作者: Min-Guan Lin
    Bo-En Chen
    Wan-Chi Liang
    Wei-Mou Chou
    Jiau-Hua Chen
    Lih-Ying Kuo
    Long-Liu Lin
    貢獻者: 食品科技系
    關鍵字: Bacillus licheniformis
    GrpE
    Site-saturation mutagenesis
    Co-chaperone activity
    Temperature-induced denaturation
    日期: 2010-06
    上傳時間: 2012-05-22 13:53:31 (UTC+8)
    摘要: To elucidate the role of leucine 134 of Bacillus licheniformis nucleotide exchange factor (BlGrpE), site-saturation mutagenesis was employed to generate all possible replacements for this residue. Wild-type and mutant proteins were purified by nickel-chelated chromatography and had a molecular mass of approximately 34.5 kDa. As compared with wild-type BlGrpE, the nucleotide exchange factor (NEF) activity of L134H, L134K, L134R, L134D, L134E, L134N, L134Q, L134S, L134G and L134P was reduced by more than 96%. In vitro binding assay revealed that wild-type BlGrpE and the functional variants mainly interacted with the monomer of BlDnaK, but no such interaction was observed for the remaining mutant proteins. BlGrpE and 9 mutant proteins synergistically stimulated the ATPase activity of B. licheniformis DnaK (BlDnaK), whereas the NEF-defective variants had no synergistic stimulation. Comparative analysis of the far-UV CD spectra showed that the α-helical content of the inactive mutant BlGrpEs was reduced significantly with respect to wild-type protein. Moreover, the inactive mutant proteins also exhibited a more sensitivity towards the temperature-induced denaturation. Taken together, these results indicate that Leu134 might play a structural role for the proper function of BlGrpE.
    显示于类别:[ 食品科技系 ] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    99_63_j.pdf685KbAdobe PDF207检视/开启
    index.html0KbHTML384检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈