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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/25229


    標題: RGNNV induces mitochondria-mediated cell death via newly synthesized protein dependent pathway in fish cells
    作者: Horng-Cherng Wu
    Jen-Leih Wu
    Heuy-Ling Chu
    Yu-Chin Su
    Jiann-Ruey Hong
    貢獻者: 食品科技系
    關鍵字: Nervous necrosis virus
    Necrotic cell death
    Mitochondria
    RNA viral genome
    RNA interference
    Viral death inducer
    日期: 2010-09
    上傳時間: 2012-05-22 13:53:30 (UTC+8)
    摘要: The RNA nervous necrosis virus induces necrotic cell death in fish; however, the molecular mechanism remains unknown. In this study, we demonstrated that beta-nodavirus-induced mitochondria-mediated dependent cell death is through newly synthesized protein dependent pathway in replication cycle. We determined that newly synthesized protein dependent pathway is required for red-spotted grouper nervous necrosis virus (RGNNV)-induced cell death. UV irradiation of the virus effectively blocked viral replication and cell death. Next, RGNNV RNA-dependent RNA polymerase (RdRp or protein A) was cloned and its involvement in RNA genome replication and viral protein synthesis was analyzed. Protein A was initially expressed 48 h post-infection and localized to the cytoplasm. Knockdown of protein A expression completely blocked viral genomic replication and expression of viral protein expression RNA1 small hairpin RNA (shRNA) producing cell lines, which coincided with inhibition of phosphatidylserine exposure, mitochondria-mediated death signaling, and increased cell viability 72 h post-infection. Furthermore, RGNNV-induced mitochondria-mediated caspase-3-independent necrotic cell death is dependent on viral synthesized protein dependent pathway at the middle-late replication stage. Taken together, for instance these results suggested that RGNNV induces cell death may require newly synthesized protein for triggering host mitochondria-mediated cell death. These findings may provide new insights into RNA viral pathogenesis.
    關聯: Fish & shellfish immunology 29(3):p.451-463
    Appears in Collections:[ 食品科技系 ] 期刊論文

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