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    Title: A Recombinant Metalloprotease Antigen of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model
    Authors: Y.-C. Chen
    C.-C. Chang
    S.-Y. Chang
    J.-H. Su
    Contributors: 生物科技系
    Keywords: infection
    protective effect
    Vibrio vulnificus
    Date: 2010-02
    Issue Date: 2012-03-30 15:17:44 (UTC+8)
    Publisher: Wiley-Blackwell
    Abstract: Aims:  To investigate whether Vibrio vulnificus metalloprotease (VvpE) can induce the production of specific anti-VvpE antibody to confer effective protection against Vibrio vulnificus infection and to evaluate the possibility of VvpE as a potential vaccine candidate against disease caused by V. vulnificus.

    Methods and Results:  The gene encoding the 65-kDa VvpE of V. vulnificus was amplified by PCR and cloned into the expression vector pET21(b). The recombinant VvpE of V. vulnificus was expressed in Escherichia coli BL21(DE3). This His6-tagged VvpE was purified and injected intramuscularly into mice to evaluate its ability to stimulate immune response. Specific antibody levels were measured by ELISA. The 75% protective efficacy of recombinant VvpE was evaluated by active immunization and intraperitoneal challenge with V. vulnificus in mice.

    Conclusions:  The recombinant His6-tagged VvpE of V. vulnificus is capable of inducing high antibody response in mice to confer effective protection against lethal challenge with V. vulnificus. VvpE might be a potential vaccine candidate to against V. vulnificus infection.

    Significance and Impact of the Study:  This study uses His6-tagged VvpE to act as vaccine that successfully induces effective and specific anti-VvpE antibody and offers an option for the potential vaccine candidate against V. vulnificus infection.
    Relation: Letters in Applied Microbiology 50(2):p.168-172
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles
    [Dept. of Food Science & Technology] Periodical Articles

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