English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18033/20233 (89%)
造訪人次 : 23240635      線上人數 : 282
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/25057


    標題: Molecular Cloning mzd Structural Analysis of Murine Thymidine Kinase Genomic and cDNA Sequences
    作者: PIN-FANG LIN
    HOWARD B. LIEBERMAN
    DONG-BOR YEH
    TIAN XU
    SHOU-YUAN ZHAO
    FRANK H RUDDLE
    貢獻者: 食品衛生科
    日期: 1986
    上傳時間: 2012-02-24 16:04:41 (UTC+8)
    摘要: Two functional cytosolic thymidine kinase (tk) cDNA clones were isolated from a mouse L-cell library An RNA blot analysis indicated that one of these clones contains a nearly full-length tk sequence and that LTK ̄cells contain little or no TK message. The nucleotide sequences of both clones were determined, and the functional mouse tk cDNA contains 1,156 base pairs. An analysis of the sequence implied that there is an untranslated 32-nucleotide region at the 5 end of the mRNA, followed by an open reading frame of 699 nucleotides. The 3 untranslated region is 422 nucleotides lonpg. Thus , the gene codes for a protein containing 233 amino acids, with a molecular weight of 25,873. A comparison of the coding sequences of the mouse tk cDNA with the human and chicken tk genes revealed about 86 and 70% homology, respectively. We also isolated the tk gene from a mouse C57BL/10J cosmid library. The structural organization was determined by a mouse tk cDNA. The tk gene spans approximately 11 kilobases and contains at least five introns. Southern blot analysis revealed that this gene is deleted in mouse LTK ̄cells, consistent with the inability of these cells to synthesize TK message. This analysis also shovved that tk-related sequences are present in the genomes of several mouse strains, as well as in LTK-cells. These segments may represent pseudogenes.
    關聯: 嘉南學報 12:p.A69-A76 轉載 Molecular and Cellular Biology p.3149-3156 (Nov. 1985)
    顯示於類別:[嘉南學報] 12期 (1986)
    [ 食品科技系 ] 期刊論文

    文件中的檔案:

    沒有與此文件相關的檔案.



    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋