本計畫擬研究含硫雜環之化妝品與藥品中四氢噻唑衍生物測定與快速檢測器之設計，其目標如下：第一年：分別電沉積各種金屬薄膜在白金、金與銀之石英晶體(QCM)當工作電極，利用各種光譜法研究這些奈米混合電極。製作流動式電解槽-連接石英晶體感測器(QCM flowcell)，連線電化學與石英晶體微天平儀器(EQCM-flow cell)，此系統用於研究藥物的吸附動力學與含量測定。評估各種參數如電解質、溫度與石英晶體粗糙面，對石英晶體在流動式電解槽之影響。此系統可同時獲得藥物的電位、電流、頻率變化值，與電流對時間關係圖。將QCM 感測器與紫化光感測器串聯於液相層析儀，測定化妝品與藥品中四氢噻唑衍生物含量。第二年：利用各種電合成方法，在適當條件下電解氧化金屬或其鹽類，合成奈米結構之金屬氧化物電極(如氧化鎂、氧化金、氧化銀與氧化鉛)當陽電極材料。研究各種反應條件(溶液組成、使用電位與時間)對電極材料形態之影響。以伏特安培法與EQCM 法等研究合成產物的特性與電化學性質。並比較這些電極對四氢噻唑衍生物的感度與特異性。第三年：利用電化學交流阻抗光譜法(EIS)與循環伏特安培法(CV)，研究四氢噻唑衍生物在奈米結構金屬氧化物修飾碳電極之電化學行為。並與未修飾碳電極作比較。快速測得電容與時間關係圖，鑑定藥物成分。測得濃度變化與電容差值(△C)之線性圖，計算出藥物之解離常數與濃度。 The project is proceeding with the construction study of sulphur- containing heterocyclic thiazolidine derivatives in cosmetics and drugs. The objects are the following as: First year: Various metals film electrodeposits on Pt, Au and Ag/quartz crystal resonator of an electrochemical quartz crystal microbalance (EQCM) is designed, fabricated and characterized, respectively. Design a flow – through quartz crystal microbalance sensor for measure and adsorption studies. The response of the quartz crystal to different parameters such as electrolyte, temperature and quartz roughness in the flow cell was evaluated. The system performance was tested under different flow conditions. Simultaneous measurements of resonant frequency change and potential-current or current-time transients allowed investigated of thiazolidine derivatives electrochemical processes. The QCM apparatus is arranged before the UV detectors of liquid chromatography apparatus in parallel alignment. Finally, the flow cell- EQCM analysis is used for the determination thiazolidine derivatives in commercial cosmetic and drug products. Second year: Nano-structured of metal oxides, (e;g. Mg oxide, Au oxide, Ag oxide and Pb oxide) are synthesized by electrochemical oxidation of metals and its salts under the controlled conditions, as anode materials. The influence of different reaction conditions which solution component, applied voltage and time on the morphology of the products are studied. The synthesis, characterization, and electrochemical properties of metal oxides are investigated to enhance the anodic properties concurrent use of voltammatry and EQCM methods. The sensitivity and specificity of these materials are used to determination of metabolites of the thiazolidine derivatives are compared. Third year: Electrochemical behavior of thiazolidine derivatives on a carbon-coated with metal oxide films modified electrodes are studied by electrochemical impedance spectroscopy (EIS) nd cyclic voltammatry (CV). Also, compare with pure nanosized metal oxides, and nanoparticles modified carbon paste electrode. The time profile of capacitance is used to fast in situ monitoring thiazolidine derivatives. The relationship between decrease of capacitance (△C) and the logarithm of thiazolidine concentration is measured with EIS and used for calculation of association constants and quantitication.