本研究以離子液體結構為基礎，開發出8種雙陽離子聚離子液體，接著經由(1)紙錠擴散試驗(disc diffusion assay)與(2)最低抑菌濃度(minimal inhibitory concentration, MIC)及最低殺菌濃度(minimal microbicidal concentration, MMC)之方法評估抗菌性能，探討聚離子液體中不同的陽離子結構對抗菌效能之影響。
由紙錠擴散試驗結果顯示雙陽離子聚離子液體(26a-26h)對革蘭氏陽性菌-金黃色葡萄球菌及真菌-白色念珠菌的抑制效果較雙陽離子離子液體單體(25a-25h)佳，推測其原因可能與聚合物的電荷密度較單體高，有助於與菌體的吸附作用，以利抗菌作用。針對白色念珠菌之最小抑菌濃度(MIC)及最小殺菌濃度(MMC)雙陽離子聚離子液體化合物26h有最佳的最低抑菌濃度110μg/mL及最低殺菌濃度131.7μg/mL。 This thesis is based on the structures of ionic liquids to develop eight types of dicationic poly(ionic liquid)s. And then the antimicrobial efficacy is evaluated by the methods of (1) disc diffusion assay, (2) MIC (minimal inhibitory concentration) and MMC (minimal microbicidal concentration) to investigate the antimicrobial efficacy affected by alkyl chain with different types of cations.
The results of disc-diffusion assay display that the dicationic poly(ionic liquid)s (25a-25h) show better antibacterial performance for Gram-positive bacteria (S.aureas) and fungi (C. albicas) than dicationic ionic liquid monomers (25a-25h). the possible factor is that the charge density of poly(ionic liquid)s is higher than their corresponding monomers and contributes the adsorption interaction with bacteria to execute the antibacterial performance. Dicationic poly(ionic liquid)s (26h) has the best minimum inhibitory concentration for studying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MMC) of the fungi (C. albicas). The values of minimum inhibitory concentration is respectively 110μg/mL (26h), and the values of minimum bactericidal concentration are also respectively 131.7μg/mL (26h).
