台灣位處於亞熱帶，每年曝曬在中上強度紫外線下的季節長達半年以上，因此，只要參予戶外活動超過二十分鐘就很容易曬黑和曬傷。在如此環境之下如何保護自己避免皮膚的傷害將是一個很重要的課題。傳明酸(tranexamic acid)，以往常用來做為血小板的代替品，使用在手術中或流血不止時的斷血藥物。而近幾年來被發現在減少麥拉寧色素方面有顯著的效果，因此使用於開發在化粧品美白方面的產品。
化粧品的有效性經皮吸收是一個主要的研究方向，本研究是以電化學方式及呈色分析法以偵測出分析物的含量，與其常見的分析方法相比具有快速、減量、靈敏度高及穩定等優點，加上容易攜帶、使用的耗材量少，常使用在微量的分析物的分析。
本研究的目的在於使用電化學方式分析傳明酸的安定性及呈色分析法分析經皮吸收的滲透量和滲透速度，以評估化粧品配方中含有傳明酸做為美白成分時，配方開發的參考。
研究結果顯示，傳明酸(tranexamic acid)在體外經皮吸收實驗中有經皮吸收效果。其中添加促進劑的效果又大於未添加促進劑者。在添加促進劑方面，在經皮吸收24小時後的測試發現，添加丙二醇(Propylene glycol ; PG)促進劑是未添加的4.3倍，添加甘油(Ｇlycerin)是未添加者的5.07倍，添加1,3丁二醇(1,3-Butylene glycol ; 1,3-BG)則是未添加的3.45倍，故以添加甘油者最佳。本研究藉由體外經皮吸收所得到經皮吸收之訊息可作為配方開發之設計與研究。
關鍵字：傳明酸、電化學、呈色分析法、經皮吸收 The weather in Taiwan is so hot during the summer time. So Taiwanese pepole who wanted to avoid getting skin damage with strong ultraviolet light in the summer season. Using the Skin whitening in Taiwan is a very important issue. There are many kind of Skin whitening agents usually applying on Cosmetics Industry such as Tranexamic acid, Viamine C, and. Arbuin etc. Tranexamic acid was choose in this study because of it's a kind of more stable compound among the whitening agents. Originally it is one of efficient and very usual using compound in platelet substitute products.
The Colorimetric analysis method and the electrochemistry methord were applied for quantitive test of Tranexamic acid after the percutaneous absorption experiments has been performed. The Colorimetric analysis method is rarely used on percutaneous absorption experiments to assy the amound of the compounds which were pass through the skin membrans. Tranexamic acid is a kind of amino acid. According to the lectures recently amino acid is assied usually by Colorimetric analysis method. Four kind of products has been prepared one is a product including tranexamic acid only. The other are including tranexamic acid and a kind of percutaneous absorption promoting agent respectively. There are three kinds of percutaneous absorption promoting agents including Propyl Glycol(PG), Glycerin, and 1,3-Butylene glycol(1,3-BG). The pig skin membran was choose to perform percutaneous absorption here. The results showed that the product without Promoting agent has been tested to spent a lot of time when the peak is apparently on the applying Colorimetric analysis method. The absorption peak at the wave lenghth 570nm was applied on Colorimetric analysis to assy the amound of passed tranexamic acid. We found that among the percutaneous absorption promoting agents could help tranexamic acid went thrugh the skin membrans.
Based on the results of among the producs which include promoting agents showed that the ratio to the amounds of tranexamic acid of the product without promoting agent, the product including PG is 4.3 times, including Glycerin is 5.07 times, and 1,3 BG is 3.45 times of on the photoabsorption tests respectively. And glycerin is the best promoting agent. Because it has needed the showest time to monitor and has assied the largest amound of the passed tranexamic acid was detected.
Keywords: tranexamic acid, electrochemical, Colorimetric analysis, percutaneous absorption
