溫泉醫學美容的研究證實，溫泉中豐富的化學成分對於皮膚有保護、舒緩、抗自由基等功效。本研究的目的是為了解溫泉對於皮膚的美容效益，並透過細胞和人體皮膚實驗評估，進一步探討溫泉的美容效益作用機轉。本研究是利用碳酸氫鹽泉，評估碳酸氫鹽泉於溫泉美容效益上相關機制的探討。以B-16小鼠黑色素瘤細胞為實驗材料，發現碳酸氫鹽泉具有保護細胞的能力，在加入過氧化氫自由基24小時後細胞存活率達74.14±1.79 %，48小時後細胞存活率還有72.68±6.01%，與對照組PBS比較後在統計上達顯著差異。B-16小鼠黑色素瘤細胞釋放黑色素的實驗中，碳酸氫鹽泉的單一細胞黑色素生成率為0.914±0.061，比對照組PBS 的單一細胞黑色素生成率1.049±0.055低，且在統計上達顯著差異，發現碳酸氫鹽泉能清除自由基，減緩黑色素生成。於皮膚美容效益上，碳酸氫鹽泉使用在肌膚上於膚色改善上與對照組生理食鹽水，在統計上達顯著差異，發現碳酸氫鹽泉能增加肌膚明亮度及降低對皮膚刺激或過敏情形，對皮膚有舒緩作用。藉由此研究，推論碳酸氫鹽泉應用於溫泉美容效益上，有少許的功效。本研究僅利用碳酸氫鹽泉做為實驗的樣本，如能進一步針對各種不同泉質所含的化學成分對美容效益之探討，為溫泉美容相關產品進行研發，期望於溫泉妝品有更大的貢獻，推動溫泉相關產業的蓬勃發展。 The medical research had confirms that spring water contents rich chemical elements and had a lot of benefits for human health, such as skin protection, smoothing and anti-free radical effect. The purpose of this study was to understand the benefits of spring water to skin care by the evaluative research of cell protection and human skin care. The hydrogen carbonate spring water was used in this study to assess the effectiveness of skin care and the relevant mechanisms. In this study, B-16 mouse melanoma cells as experimental material, and the free hydroxyl radical was generated by hydrogen peroxide to induced cell death or melanin formation. Co-culture with hydrogen peroxide free radical in the 24 hours, the cell survival rate were 74.14 � 1.79% and in 48 hours later cell survival were 72.68 � 6.01%, which compared with the PBS control group had a statistically significant. In the experiment of B-16 mouse melanoma cells to evaluate the single cell release rate of melanin, the hydrogen carbonated spring water was able to slow down melanin production (0.914 � 0.061) that compared with PBS controls melanin production rate (1.049 � 0.055), had statistically significant. The results was found that hydrogen carbonate spring water has the ability to scavenging free radicals, to protect cells, to increase cell survival ratio, and slow down the melanin production. The evaluation studied in dermatology had shown that hydrogen carbonate spring water was able to increase the skin brightness, lower irritation and relief the allergic conditions.
Through this research that hydrogen carbonate spring water was produced the cell protective and cosmetic effects for skin care. However, in this study, only the use of hydrogen carbonate spring water as the experimental samples to evaluate the effects of skin care, furthermore, to understand the different hot springs on the effectiveness of skin care will be discussion on the other research. The results also suggested that the hydrogen carbonate spring water may be suitable materials to development related products of cosmetics. The results may expect a greater contribution to promote the rapid development of spa related industries.