Interaction Between Surfactin and U-937 Human Macrophages: Cytotoxicity, Reactive Oxygen Species Content, Mitochondria Membrane Potential, Cell Cycle Profiles, and in vitro Proteolytic Stability

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Title:	Interaction Between Surfactin and U-937 Human Macrophages: Cytotoxicity, Reactive Oxygen Species Content, Mitochondria Membrane Potential, Cell Cycle Profiles, and in vitro Proteolytic Stability
Authors:	Jung-hua Steven Kuo (郭榮華) Jingyueh Jeng (鄭靜月) Meng-jie Lioua (劉孟捷)
Date:	2011
Issue Date:	2011-06-23 14:57:47 (UTC+8)
Abstract:	Surfactin is a biosurfactant widely used in biomedical applications; however, information on its biocompatibility and stability in humans is limited. Surfactin is hemolytic, and because it is a peptide, its in vivo proteolytic stability must be validated for use in humans. Similar to particulate foreign bodies, surfactin is captured primarily by the human mononuclear phagocyte system. The interactions between surfactin and macrophages are important because macrophages are central in the host defense system and provide opportunities for evaluating the cytotoxicity of surfactin. Hence, we examined the changes in human macrophages (U-937 cells) after they had been treated with surfactin: their dehydrogenase activity, an indicator 01 cell viability, and their intracellular responses- reactive oxygen species content, mitochondrial membrane potential, and cell cycle profiles- were assessed. The in vitro stability of surfactin was analyzed using MALDI-TOF (matrix-assisted laser desorption and ionization with time-of-flight mass spectrometry. Surfactin had dose-dependent toxic effects on U-937 cell viability and damaged surfactin-treated HeLa (human cervical cancer) and NIH/3T3 (mouse fibroblast) cells. Hydrogen peroxide production in surfactin-treated macrophages was unstable. However, superoxide anion content significantly increased relative to that in untreated control cells over the incubation times (0.5 and 1 h). Mitochondrial membrane potential rose dose-dependently relative to that in untreated control cells, except for significant increases at a dose of 5 uM. Apoptosis developed at higher doses after 4 h of incubation at 10 uM. Surfactin was stable for -24 h of incubation. Our findings may provide a better understanding of the action of surfactin in humans.
Relation:	2011年台俄有機、藥物與生物化學交流暨藥物化學研討會，起迄日：2011/04/06~2011/04/05，地點：溪頭台大實驗林
Appears in Collections:	[Pharmacy and Science] 2011 Taiwanese-Russian Organic, Medicinal and Bio Chemistry Interactions & PST Medicinal Chemistry Symposium [Dept. of Pharmacy] Proceedings [Dept. of Biotechnology (including master's program)] Proceedings

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