Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/24230
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18057/20255 (89%)
Visitors : 1484415      Online Users : 662
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/24230


    Title: Development of an Universal Anti-Polyethylene Glycol RenorterGene for Noninvasive Imaging of PEGylated Probes
    Authors: Kuo-Hsiang Chuang (莊國祥)
    Yu-Ling Lu (呂玉玲)
    Steve Roffler (羅傅倫)
    Tian-Lu Cheng (鄭添祿)
    Date: 2011
    Issue Date: 2011-06-23 14:57:20 (UTC+8)
    Abstract: Development of a highly specific and non-immunogenic reporter to monitor gene expression in vivo is critical for successful optimization ot gene and cell therapy protocols. Here we developed a membrane-anchored anti-polyethylene glycol (aPEG) eporter that can specifically bind PEGylated imaging probes to assess the location, extent and persistence of gene expression or transplanted cells in vivo. Functional aPEG reporters that were stably expressed on cells in vitro and in vivo selectively accumulated various PEGylated imaging probes and could be detected by optical imaging, magnetic resonance (MR) imaging and micro-positron emission tomography (micro-PET). The aPEG reporter displayed an imaging specificity comparable to HSV-tk but did not provoke immune responses or cause toxicity to the host. Importantly, a humanized aPEG reporter retained high imaging speciiicuy in subcutaneous and metastatic tumor models in vivo. Thus, the highly specific and non-immunogenic aPEG reporter may be paired with PEGylated probes to pro'ovide a valuable system to image gene expression or cell delivery in the clinic.
    Relation: 2011年台俄有機、藥物與生物化學交流暨藥物化學研討會,起迄日:2011/04/06~2011/04/05,地點:溪頭台大實驗林
    Appears in Collections:[Pharmacy and Science] 2011 Taiwanese-Russian Organic, Medicinal and Bio Chemistry Interactions & PST Medicinal Chemistry Symposium
    [Dept. of Pharmacy] Proceedings

    Files in This Item:

    File Description SizeFormat
    pp-14.pdf70KbAdobe PDF574View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback