本研究利用高效液相層析法可將乙醯水楊酸，水楊酸，普卡因醴肢， N一乙醴普卡因醯胺等藥品同時予以分離及定量，其分析條件為Hibar Lichrosorb RP-18 (5um;長25 cm)的層析管，波長為280 nm的紫外光偵測器，1ml/min的流速及含15%氰甲烷，0.1%三乙胺， 4%醋酸， 0.4%醋酸鈉， pH為3.2水溶液的移動相。
在上述測定條件下，各化合物滯留時間為普卡因醯胺3.56分鐘，己醴普卡因醯胺4.37分鐘，咖啡因(內部標準品) 5.94分鐘，水楊酸10.63分鐘，已醴水楊酸14.46分鐘。在利用波峰高度計算法計算後發現藥品濃度和吸收峰高度比值呈良好的線性關係，同時所作的日內及日間精確性試驗的變異係數均小於2%，證明本測定方法之精確性良好。 A simple and sensitive high performance Hquid chromatorgraphy (HPLC) method has been established for the separation and quantification of Aspirin. Salicylic acid, Pro-cainamide and N_acetylprocainamide. The conditions of HPLC system were as followings:
(1) Column: Hibar Lichrosorb RP-18 (5 μm)
(2) Detector: UV 280 nm
(3) Mobile phase: An aqueous solution containing 15% acetonitrile, O.1% triethylamine,4% acetic acid and O.4% sodium acetate (pH = 3.2)
(4) Flow rate: 1 ml/min
nder these condations, the retention times were 3.56 min, 4.37 mm, 5.94 min,10.63 min, 14.46 min for Procainamide, N-Acetylprocainamide, Caffeine (intemal standard), Salicybc acid and Aspirin, respectivelu. The quantitative analysis was preformed by using peak hight ratio method with caffeine as an internal standard, the drug com-ponents show doseresponse relationship, the interday valdition test and intraday precision test yieldeded a coefficient of variance <2%.
