百合鱗莖萃取物抗致突變性及抗菌性研究

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Title:	百合鱗莖萃取物抗致突變性及抗菌性研究 Antibacterial and Antimutagenic Properties of Extract from Lily Bulb
Authors:	康志強王櫻娟邱淑媛
Contributors:	保健營養系
Keywords:	百合Lilium lancifolium Thunb 安氏試驗抗致突變性抑制環抗菌 Lilium lancifolium Thunb Ames test Mutagenicity Antimutagenicity Inhibition zone Antibacteria
Date:	2009-10
Issue Date:	2010-11-12 16:11:21 (UTC+8)
Abstract:	本研究以虎皮百合(Lilium lancifolium Thunb)鱗莖粉末為材料，探討溶劑萃取物之抗菌性及抗致突變性。濾紙鍵擴散試驗顯示，丙酮及正己烷萃取物無抗菌能力，乙醇萃取物對Escherichia coli DH5a、Bifidobacterium longum BCRC 14602與Lactobacillus plantarum BCRC 10357不其抗菌性，但對Bacillus thuringiensis BCRC 11501、Bacillus thuringiensis BCRC 15860、Bacillus subtilis HN9804、Bacillus natto HN9501、Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651與Streptococcus thermophilus BCRC 12257具抗菌性。對Bacillus thuringiensis BCRC 11501之最低抑菌劑量為2.67 mg/disk，抑菌物質具熱安定性。以鼠傷寒沙門氏菌(Salmonella typhimurium)TA98及TA100菌株進行安氏試驗(Ames test)，結果顯示百合鱗莖萃取物在0.1-10 mg/plate濃度下，不論是否添加鼠肝S9混合物均不具致突變性，且對benzo(a)pyrene (B[a]P)及4-nitroquinoline l-oxide (4-NQO)所誘導之突變具有抑制作用。另外，在白血球活化試驗中發現百合鱗莖乙醇萃取物不具活化白血球吞噬細菌之功能。 In the present study, the antibacterial activity and antimutagenecity of extracts obtained from Lilium lancifolium Thunb were examined. The acetone extract and hexane extract had no antibacterial activity Inhibition zones of the ethanol extract against Bacillus thuringiensis BCRC 11501, B. thuringiensis BCRC 15860, B. subtilis HN9804, B. natto HN9501, Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651 and Streptococcus thermophilus BCRC 12257 were observed. The antibacterial component of the ethanol extract was thermostable. The minimal inhibition dose of the extract against B. thuringiensis BCRC 11501 was 2.67 mg/disk. Results from the Ames test with Salmonella typhimurium TA98 and TA100 demonstrated that the ethanol extract in the concentrations of 0.1~10 mg/plate exerted no mutagenicity. Furthermore, it could suppress the base pair-substitution mutations generated by both benzo (a) pyrene (B[a]P) and by 4-quinoline 1-oxide (4-NQO). The ethanol extract was unable to activate the phagocytotic activity of polyrnorphonuc1ear leukocytes (PMN) against S. aureus BCRC 12651. In the present study, the antibacterial activity and antimutagenecity of extracts obtained from Lilium lancifolium Thunb were examined. The acetone extract and hexane extract had no antibacterial activity Inhibition zones of the ethanol extract against Bacillus thuringiensis BCRC 11501, B. thuringiensis BCRC 15860, B. subtilis HN9804, B. natto HN9501, Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651 and Streptococcus thermophilus BCRC 12257 were observed. The antibacterial component of the ethanol extract was thermostable. The minimal inhibition dose of the extract against B. thuringiensis BCRC 11501 was 2.67 mg/disk. Results from the Ames test with Salmonella typhimurium TA98 and TA100 demonstrated that the ethanol extract in the concentrations of 0.1~10 mg/plate exerted no mutagenicity. Furthermore, it could suppress the base pair-substitution mutations generated by both benzo (a) pyrene (B[a]P) and by 4-quinoline 1-oxide (4-NQO). The ethanol extract was unable to activate the phagocytotic activity of polyrnorphonuc1ear leukocytes (PMN) against S. aureus BCRC 12651.
Relation:	台灣農業化學與食品科學 47(5)：p.221-227
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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