English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17423/19718 (88%)
Visitors : 6231017      Online Users : 798
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/23209


    標題: 百合鱗莖萃取物抗致突變性及抗菌性研究
    Antibacterial and Antimutagenic Properties of Extract from Lily Bulb
    作者: 康志強
    王櫻娟
    邱淑媛
    貢獻者: 保健營養系
    關鍵字: 百合Lilium lancifolium Thunb
    安氏試驗
    抗致突變性
    抑制環
    抗菌
    Lilium lancifolium Thunb
    Ames test
    Mutagenicity
    Antimutagenicity
    Inhibition zone
    Antibacteria
    日期: 2009-10
    上傳時間: 2010-11-12 16:11:21 (UTC+8)
    摘要: 本研究以虎皮百合(Lilium lancifolium Thunb)鱗莖粉末為材料,探討溶劑萃取物之抗菌性及抗致突變性。濾紙鍵擴散試驗顯示,丙酮及正己烷萃取物無抗菌能力,乙醇萃取物對Escherichia coli DH5a、Bifidobacterium longum BCRC 14602與Lactobacillus plantarum BCRC 10357不其抗菌性,但對Bacillus thuringiensis BCRC 11501、Bacillus thuringiensis BCRC 15860、Bacillus subtilis HN9804、Bacillus natto HN9501、Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651與Streptococcus thermophilus BCRC 12257具抗菌性。對Bacillus thuringiensis BCRC 11501之最低抑菌劑量為2.67 mg/disk,抑菌物質具熱安定性。以鼠傷寒沙門氏菌(Salmonella typhimurium)TA98及TA100菌株進行安氏試驗(Ames test),結果顯示百合鱗莖萃取物在0.1-10 mg/plate濃度下,不論是否添加鼠肝S9混合物均不具致突變性,且對benzo(a)pyrene (B[a]P)及4-nitroquinoline l-oxide (4-NQO)所誘導之突變具有抑制作用。另外,在白血球活化試驗中發現百合鱗莖乙醇萃取物不具活化白血球吞噬細菌之功能。
    In the present study, the antibacterial activity and antimutagenecity of extracts obtained from Lilium lancifolium Thunb were examined. The acetone extract and hexane extract had no antibacterial activity Inhibition zones of the ethanol extract against Bacillus thuringiensis BCRC 11501, B. thuringiensis BCRC 15860, B. subtilis HN9804, B. natto HN9501, Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651 and Streptococcus thermophilus BCRC 12257 were observed. The antibacterial component of the ethanol extract was thermostable. The minimal inhibition dose of the extract against B. thuringiensis BCRC 11501 was 2.67 mg/disk. Results from the Ames test with Salmonella typhimurium TA98 and TA100 demonstrated that the ethanol extract in the concentrations of 0.1~10 mg/plate exerted no mutagenicity. Furthermore, it could suppress the base pair-substitution mutations generated by both benzo (a) pyrene (B[a]P) and by 4-quinoline 1-oxide (4-NQO). The ethanol extract was unable to activate the phagocytotic activity of polyrnorphonuc1ear leukocytes (PMN) against S. aureus BCRC 12651.
    In the present study, the antibacterial activity and antimutagenecity of extracts obtained from Lilium lancifolium Thunb were examined. The acetone extract and hexane extract had no antibacterial activity Inhibition zones of the ethanol extract against Bacillus thuringiensis BCRC 11501, B. thuringiensis BCRC 15860, B. subtilis HN9804, B. natto HN9501, Salmonella enterocolitica BCRC 12948, Staphylococcus aureus BCRC 12651 and Streptococcus thermophilus BCRC 12257 were observed. The antibacterial component of the ethanol extract was thermostable. The minimal inhibition dose of the extract against B. thuringiensis BCRC 11501 was 2.67 mg/disk. Results from the Ames test with Salmonella typhimurium TA98 and TA100 demonstrated that the ethanol extract in the concentrations of 0.1~10 mg/plate exerted no mutagenicity. Furthermore, it could suppress the base pair-substitution mutations generated by both benzo (a) pyrene (B[a]P) and by 4-quinoline 1-oxide (4-NQO). The ethanol extract was unable to activate the phagocytotic activity of polyrnorphonuc1ear leukocytes (PMN) against S. aureus BCRC 12651.
    關聯: 台灣農業化學與食品科學 47(5):p.221-227
    Appears in Collections:[保健營養系(所) ] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML752View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback