Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/23142
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/23142


    Title: Noninvasive optical imaging for tracking gene delivery and recombination in tumor
    Authors: Mei-Ru Chen
    Vivia Yu-Ying Kao
    Shan-Wen Liu
    Tzu-Chin Wu
    Hsiao-Chi Yu
    Chin-Han Hsu
    Chin-Tu Chen
    Kurt M. Lin
    Contributors: 生物科技系
    Keywords: Green fluorescence protein
    Bioluminescence
    Firefly luciferase
    Cre recombinase
    loxP site
    Date: 2009-10
    Issue Date: 2010-10-27 14:14:29 (UTC+8)
    Publisher: World Scientific Publ Co Pte Ltd
    Abstract: Here, we report the generation of optical imaging reporter breast tumor cells that allow the longitudinal, in vivo, noninvasive imaging of gene recombination in tumor. Tumor-gene targeting is a promising approach of treating cancers, and a suitable gene delivery method is the criteria for success. By using the cre lox genetic engineering tool, we targeted stable green fluorescent protein expression in metastatic-prone human breast cancer MDA-MB231 cells that switch to express firefly luciferase upon the exogenous delivery and expression of cre DNA recombinase. We tested this model in vivo by intratumor injection of cre adenovirus and demonstrated the usefulness of this model to achieve longitudinal bioluminescence imaging of DNA recombination in tumor. This optical imaging vector and tumor model will facilitate the research for biomaterial solutions for carriers in gene therapy, and in studies on tumor targeting, tracking for tumor metastasis and migration of tumor stem cells, and for determining the anticancer drug efficacy.
    Relation: Biomedical Engineering Applications, Basis and Communications 21(5)：p.355-360
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

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